Compositions, methods, and systems for bead formation using improved polymers
First Claim
1. A gel, comprising:
- (a) a cell, a nucleus of said cell, or one or more constituents derived from said cell;
(b) two or more polymers, wherein a polymer of said two or more polymers comprises at least one member selected from the group consisting of a polyolefin, an olefin copolymer, an acrylic, a vinyl polymer, a polyamide, a polyimide, a formaldehyde resin, a polyurethane, a cellulosic, a thermoplastic elastomer, and a thermoplastic polyurethane; and
(c) a plurality of linkers, each of said plurality of linkers comprising a 1,2,3-triazole moiety, wherein said plurality of linkers crosslink said two or more polymers, and wherein said plurality of linkers is distributed throughout said gel.
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Abstract
The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.
163 Citations
49 Claims
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1. A gel, comprising:
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(a) a cell, a nucleus of said cell, or one or more constituents derived from said cell; (b) two or more polymers, wherein a polymer of said two or more polymers comprises at least one member selected from the group consisting of a polyolefin, an olefin copolymer, an acrylic, a vinyl polymer, a polyamide, a polyimide, a formaldehyde resin, a polyurethane, a cellulosic, a thermoplastic elastomer, and a thermoplastic polyurethane; and (c) a plurality of linkers, each of said plurality of linkers comprising a 1,2,3-triazole moiety, wherein said plurality of linkers crosslink said two or more polymers, and wherein said plurality of linkers is distributed throughout said gel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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12. A method of forming a gel, comprising:
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(a) providing a cell, a nucleus of said cell, or one or more constituents derived from said cell; and (b) generating said gel comprising (i) said cell, said nucleus of said cell, or said one or more constituents derived from said cell, (ii) two or more polymers, wherein a polymer of said two or more polymers comprises at least one member selected from the group consisting of a polyolefin, an olefin copolymer, an acrylic, a vinyl polymer, a polyamide, a polyimide, a formaldehyde resin, a polyurethane, a cellulosic, a thermoplastic elastomer, and a thermoplastic polyurethane, and (iii) a plurality of linkers, each of said plurality of linkers comprising a 1,2,3-triazole moiety, wherein said plurality of linkers crosslink said two or more polymers, and wherein said plurality of linkers is distributed throughout said gel. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification