Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
First Claim
1. A composition comprising a mesodermal cell prepared in vitro and an exogenous agent that inhibits the retinoic acid signaling pathway in the mesodermal cell, wherein the retinoic acid signaling pathway of the mesodermal cell is inhibited by the exogenous agent thereby promoting ventricular cardiomyocyte formation from the mesodermal cell.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention discloses novel compositions and methods for enhancing cardiac differentiation efficiency of stem cells or promoting ventricular and atrial cardiomyocytes formation from stem cells. The present invention also discloses the atrial and ventricular cardiomyocytes formed from the stem cells, and the uses of the cardiomyocytes for repairing cardiac injuries and screening for new medicaments for treating cardiac injuries.
-
Citations
24 Claims
- 1. A composition comprising a mesodermal cell prepared in vitro and an exogenous agent that inhibits the retinoic acid signaling pathway in the mesodermal cell, wherein the retinoic acid signaling pathway of the mesodermal cell is inhibited by the exogenous agent thereby promoting ventricular cardiomyocyte formation from the mesodermal cell.
- 9. A cell culture medium, which comprises a population of cells differentiated in vitro from a mesodermal cell whose retinoic acid signaling pathway has been inhibited by an exogenous agent, wherein, without enrichment or isolation, at least 60% of the cells in the population are cardiomyocytes, and wherein at least 80% of the cardiomyocytes are ventricular cardiomyocytes.
Specification