Polynucleotide capture materials, and methods of using same
First Claim
1. A method for nucleic acid isolation comprising:
- receiving into a process chamber a biological sample containing nucleic acid;
contacting the biological sample with a plurality of binding particles in the process chamber, the binding particles being coated with PAMAM (Generation
0) dendrimer which is amide-bonded to the binding particles, wherein at least a portion of the nucleic acids in the biological sample become reversibly bonded to the binding particles;
washing the binding particles with a solution characterized by a pH less than 10 while retaining the nucleic acid on the binding particles; and
releasing a nucleic acid sample from the binding particles by contacting the binding particles with an elution solution characterized by a pH greater than 10.
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Accused Products
Abstract
Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples are provided. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. The binding particles can be coated with PAMAM (Generation 0) dendrimer which is amide-bonded to the binding particles, wherein at least a portion of the nucleic acids in the biological sample become reversibly bonded to the binding particles.
953 Citations
20 Claims
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1. A method for nucleic acid isolation comprising:
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receiving into a process chamber a biological sample containing nucleic acid; contacting the biological sample with a plurality of binding particles in the process chamber, the binding particles being coated with PAMAM (Generation
0) dendrimer which is amide-bonded to the binding particles, wherein at least a portion of the nucleic acids in the biological sample become reversibly bonded to the binding particles;washing the binding particles with a solution characterized by a pH less than 10 while retaining the nucleic acid on the binding particles; and releasing a nucleic acid sample from the binding particles by contacting the binding particles with an elution solution characterized by a pH greater than 10. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for nucleic acid isolation comprising:
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receiving into a process chamber a biological sample containing nucleic acid; contacting the biological sample with a plurality of binding particles in the process chamber, the binding particles being coated with cationic polyelectrolyte dendrimer which is amide-bonded to the binding particles, wherein at least a portion of the nucleic acids in the biological sample become reversibly bonded to the binding particles; washing the binding particles with a solution characterized by a pH less than 10 while retaining the nucleic acid on the binding particles; and releasing a nucleic acid sample from the binding particles by contacting the binding particles with an elution solution characterized by a pH greater than 10. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A composition comprising:
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carboxyl modified microparticles; and cationic polyelectrolyte dendrimer covalently bound via one or more amine groups per molecule to one or more of the carboxylic acid groups on the microparticles. - View Dependent Claims (20)
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Specification