Waveguide-based detection system with scanning light source

• US 10,590,493 B2
• Filed: 05/17/2018
• Issued: 03/17/2020
• Est. Priority Date: 03/10/2006
• Status: Active Grant

• Current Assignee: LDIP, LLC
• Original Assignee: LDIP, LLC
• Inventors: Duer, Reuven, Chang, Yun-Pei, Shastry, Ashutosh
• Primary Examiner(s): Akanbi, Isiaka O
• Application Number:
  US15/982,999
• Publication Number:
  US 20180265937A1
• Time in Patent Office: 670 Days
• Field of Search: 356311, 356432, 356246
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/0853 Movable fibre optical member, e.g. for scanning or selecting
• CPC Class Codes: G01N 2201/10 Scanning
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 6/4226 Positioning means for moving the elements into alignment, e.g. alignment screws, deformation of the mount

Abstract

The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light source allows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner.

Claims

1. A detection system for detecting a biologically active analyte molecule comprising:

a substrate comprising one or more excitation waveguides and a plurality of collection waveguides, the one or more excitation waveguides and the plurality of collection waveguides crossing to form an array of intersection regions where an excitation waveguide and a collection waveguide cross and provide optical communication with one intersection region of the array of intersection regions at each crossing, and a plurality of optical sensing sites each in optical communication with the array of intersection regions;

a scanning light source configured to generate light that is transducable by a sensor associated with at least one of the plurality of optical sensing sites, wherein the one or more excitation waveguides is arranged to propagate the light from the scanning light source to the plurality of optical sensing sites;

a detector that is in optical communication with one or more of the plurality of collection waveguides; and

an actuator for spatially translating the light emitted from the scanning light source relative to the substrate such that the light is coupled to and in optical communication with at least one of the one or more excitation waveguides of the substrate at some point along its scanning path.

2. The detection system of claim 1, wherein the one or more excitation waveguides is a single excitation waveguide.

3. The detection system of claim 1, wherein both the one or more excitation waveguides and the plurality of collection waveguides exit the substrate at a first edge of the substrate.

4. The detection system of claim 3,

wherein the scanning light source is at some point along its scanning path in optical communication with the one or more of the excitation waveguides at the first edge of the substrate;

and

wherein the detector is in optical communication with the one or more of the plurality of collection waveguides at the first edge of the substrate.

5. The detection system of claim 1, wherein the one or more excitation waveguides are curved about 90 degrees.

6. The detection system of claim 1, wherein the sensor is configured to transduce the light when the sensor interacts with the biologically active analyte molecule, wherein the sensor includes one or more of a fluorescence well or cell, an absorption cell, an interferometric sensor, a diffractive sensor and a surface plasmon resonance (SPR detector.

7. The detection system of claim 1, wherein the plurality of collection waveguides are arranged to propagate transduced light from the at least one of the plurality of optical sensing sites to the detector, wherein the detector is configured to measure the transduced light from the at least one of the optical sensing sites.

8. The detection system of claim 1, wherein the detector includes one or more elements corresponding to one or more of the plurality of collection waveguides.

9. A detection method comprising:

delivering a sample suspected of containing a biologically active analyte molecule to be detected to an optical sensing site on a substrate of a detection system, the substrate comprising one or more excitation waveguides and a plurality of collection waveguides;

spatially translating a scanning light source to a point at which the light source is in optical communication with at least one of the one or more excitation waveguides to propagate light from the scanning light source to the optical sensing site, wherein a first light wave is transducable by a sensor associated with the optical sensing site to a second light wave carried in one or more of the plurality of collection waveguides in optical communication with the optical sensing site and crossing the one or more excitation waveguides; and

detecting a measurable change in the second light wave using a detector in optical communication with the one or more of the plurality collection waveguides, wherein the measurable change in the second light wave occurs when the sensor interacts with the biologically active analyte molecule.

10. The method of claim 9, wherein the scanning light source further comprises a detector, and wherein at the point at which the light source is coupled to and in optical communication with at least one of the one or more excitation waveguides in optical communication with the optical sensing site, the detector is also coupled to and in optical communication with said at least one of the one or more excitation waveguides.

11. The method of claim 9, wherein the biologically active analyte molecule is selected from the group consisting of a nucleic acid, a protein, an antigen, an antibody, a microorganism, a gas, a chemical agent and a pollutant.

12. The method of claim 9, wherein the sensor is adapted to support an immunoassay and wherein the sensor interacting with the biologically active analyte molecule comprises an outcome of an immunoassay.

13. The method of claim 9, wherein the one or more excitation waveguides is a single excitation waveguide.

14. The method of claim 9, wherein both the one or more excitation waveguides and the plurality of collection waveguides exit the substrate at a first edge of the substrate.

15. The method of claim 14,

wherein the scanning light source is at some point along its scanning path in optical communication with the one or more of the excitation waveguides at the first edge of the substrate;

and

wherein the detector is in optical communication with one or more of the collection waveguides at the first edge of the substrate.

16. The method of claim 9, wherein the one or more excitation waveguides are curved about 90 degrees.

17. A detection system for detecting a biologically active analyte molecule comprising:

a substrate comprising one or more excitation waveguides and a plurality of collection waveguides, the one or more excitation waveguides and the plurality of collection waveguides crossing to form an array of intersection regions where an excitation waveguide and a collection waveguide cross and provide optical communication with one intersection region of the array of intersection regions at each crossing, and a plurality of optical sensing sites each in optical communication with the array of intersection regions, wherein both the one or more excitation waveguides and the plurality of collection waveguides exit the substrate at a first edge of the substrate;

a light source configured to generate light that is transducable by a sensor associated with at least one of the plurality of optical sensing sites, wherein the one or more excitation waveguides is arranged to propagate the light from the light source to the plurality of optical sensing; and

a detector that is in optical communication with one or more of the plurality of collection waveguides.

18. The detection system of claim 17, wherein the one or more excitation waveguides is a single excitation waveguide.

19. The detection system of claim 17,

wherein the light source is in optical communication with the one or more of the excitation waveguides at the first edge of the substrate;

and

wherein the detector is in optical communication with the one or more of the plurality of collection waveguides at the first edge of the substrate.

20. The detection system of claim 17, wherein the one or more excitation waveguides are curved about 90 degrees.