Serially deposited biomolecules
First Claim
1. A process of preparing two or more oligonucleotides on a microarray, comprising:
- (a) electropolymerizing a monomer on one or more microelectrodes of a microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness on at least a first microelectrode and electropolymerizing the monomer with one or more different electropolymerizing parameters to form at least a second thickness on at least a second microelectrode, where the first microelectrode and the second microelectrode are selected from the one or more microelectrodes, where the first thickness is different than the second thickness, where the one or more electropolymerizing parameters are selected from the group consisting of potential, current and time of electropolymerizing;
(b) exposing the microarray to a first solution containing a first oligonucleotide for attachment to the first thickness on the one or more electrodes; and
(c) exposing the microarray to a second solution containing a second oligonucleotide for attachment to the second thickness on the one or more electrodes, where one or both the first thickness and the second thickness facilitates detection of one or both the first oligonucleotide and the second oligonucleotide.
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Accused Products
Abstract
Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolecule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.
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Citations
20 Claims
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1. A process of preparing two or more oligonucleotides on a microarray, comprising:
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(a) electropolymerizing a monomer on one or more microelectrodes of a microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness on at least a first microelectrode and electropolymerizing the monomer with one or more different electropolymerizing parameters to form at least a second thickness on at least a second microelectrode, where the first microelectrode and the second microelectrode are selected from the one or more microelectrodes, where the first thickness is different than the second thickness, where the one or more electropolymerizing parameters are selected from the group consisting of potential, current and time of electropolymerizing; (b) exposing the microarray to a first solution containing a first oligonucleotide for attachment to the first thickness on the one or more electrodes; and (c) exposing the microarray to a second solution containing a second oligonucleotide for attachment to the second thickness on the one or more electrodes, where one or both the first thickness and the second thickness facilitates detection of one or both the first oligonucleotide and the second oligonucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A process of preparing a multiplex microarray of serially attached different oligonucleotides on a microarray, comprising:
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(a) electropolymerizing a monomer on one or more microelectrodes of the microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness on a first microelectrode and electropolymerizing the monomer with one or more different electropolymerizing parameters to form at least a second thickness on a second microelectrode, where the first microelectrode and the second microelectrode are selected from the one or more microelectrodes, where the first thickness is different than the second thickness, where the one or more electropolymerizing parameters are selected from the group consisting of potential, current and time of electropolymerizing, where the electropolymerizing solution containing the monomer does not contain any oligonucleotides; (b) exposing the microarray with a first oligonucleotide solution containing a first oligonucleotide for attachment to the first thickness on the first microelectrode; and (c) exposing the microarray to a second oligonucleotide solution containing a second oligonucleotide for attachment to the second thickness on the second microelectrode, where one or both the first thickness and the second thickness facilitates detection of one or both the first oligonucleotide and the second oligonucleotide. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification