Nanowire-coated microdevice and method of making and using the same

• US 10,596,125 B2
• Filed: 12/11/2015
• Issued: 03/24/2020
• Est. Priority Date: 12/15/2014
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Desai, Tejal A., Dinakar, Hariharasudhan Chirra, Fox, Cade B.
• Primary Examiner(s): Proctor, Cachet I
• Application Number:
  US15/536,071
• Publication Number:
  US 20170360717A1
• Time in Patent Office: 1,565 Days
• CPC Class Codes: A61K 47/32 Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers , poly(meth)acrylates, or polyvinyl pyrrolidone
• CPC Class Codes: A61K 47/34 Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers A61K47/10 takes precedence
• CPC Class Codes: A61K 9/0097 Micromachined devices; Microelectromechanical systems [MEMS]; Devices obtained by lithographic treatment of silicon; Devices comprising chips intradermal microneedle arrays A61K9/0021; MEMS in general B81B7/02
• CPC Class Codes: A61K 9/70 Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug hollow drug-filled fibres A61K9/0092
• CPC Class Codes: A61K 9/7007 Drug-containing films, membranes or sheets A61K9/0041, A61K9/0043, A61K9/006, A61K9/0063 take precedence
• CPC Class Codes: A61L 2400/12 Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: A61M 37/00 Other apparatus for introducing media into the body for reproduction or fertilisation A61B17/425; apparatus for iontophoresis or cataphoresis A61N1/30; Percutany, i.e. introducing medicines into the body by diffusion through the skin salt baths A61H33/04
• CPC Class Codes: B81B 2207/056 of static structures
• CPC Class Codes: B81B 7/00 Microstructural systems; Auxiliary parts of microstructural devices or systems
• CPC Class Codes: B81C 1/00031 Regular or irregular arrays of nanoscale structures, e.g. etch mask layer photomechanical, e.g. photolithographic, production of textured or patterned surfaces G03F7/00; lithographic processes for making patterned surfaces using printing and stamping G03F7/0002
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites

Abstract

A microdevice containing a plurality of nanowires on a biocompatible surface, and methods of making and using the same are provided. Aspects of the present disclosure include forming a plurality of microdevices on a substrate where each microdevice includes a plurality of nanowires. The nanowires may be loaded with an active agent by disposing the active agent onto the surface of the nanowires. Also provided herein are kits that include the subject microdevices.

Claims

1. A method of forming a microdevice comprising a plurality of nanowires on a biocompatible surface, comprising:

a) depositing onto a surface of a first planar biocompatible polymeric layer a second planar biocompatible polymeric layer, wherein the melting temperature of the first biocompatible polymeric layer is higher than the melting temperature of the second biocompatible polymeric layer;

b) contacting a surface of the second planar biocompatible polymeric layer with a nanoporous membrane; and

c) forming the second planar biocompatible polymeric layer into a plurality of nanowires using the nanoporous membrane as a template,

wherein the nanowires are present on only one surface of the microdevice.

2. The method of claim 1, wherein the forming the second biocompatible polymeric layer into a plurality of nanowires using the nanoporous membrane as a template comprises extruding the second biocompatible polymeric layer through the nanoporous membrane using heat.

3. The method according to claim 1, wherein the method further comprises dissolving the nanoporous membrane after forming the second biocompatible polymeric layer into a plurality of nanowires.

4. The method of claim 3, wherein the dissolving comprises dissolving the nanoporous membrane in an alkaline solution.

5. The method of claim 1, wherein the depositing step a) comprises contacting a heated first biocompatible polymeric layer with the second biocompatible polymeric layer.

6. The method of claim 1, wherein the first planar biocompatible polymeric layer comprises polymethyl methacrylate (PMMA).

7. The method of claim 1, wherein the second biocompatible polymeric layer comprises polycaprolactone (PCL).

8. The method of claim 1, wherein the nanoporous membrane is an anodized metal oxide membrane or a nanoporous silica membrane.

9. The method of claim 8 wherein the anodized metal oxide comprises aluminum, tin or titanium.

10. The method of claim 9 wherein the nanoporous membrane is a nanoporous anodized aluminum oxide (AAO) membrane.

11. The method of claim 1, wherein the method comprises providing the first biocompatible polymeric layer on a substrate prior to step a).

12. The method of claim 11, wherein the method comprises creating a micropattern in the first biocompatible polymeric layer prior to step a).

13. The method of claim 12, wherein creating the micropattern comprises using photolithography.

14. The method of claim 11, wherein the substrate is a silicon wafer.

15. The method of claim 1, wherein the method comprises creating a micropattern in the nanoporous membrane prior to step b).

16. The method of claim 1, wherein the first polymeric layer has an average thickness in the range of 1 to 100 μ

m.

17. The method of claim 1, wherein the second biocompatible polymeric layer has an average thickness in the range of 1 to 100 μ

m.

18. The method of claim 1, wherein the average diameter of the pores of the nanoporous membrane is in the range of 10 to 500 nm.