Feedback system for parallel droplet control in a digital microfluidic device

• US 10,596,572 B2
• Filed: 08/22/2017
• Issued: 03/24/2020
• Est. Priority Date: 08/22/2016
• Status: Active Grant

• Current Assignee: Miroculus Inc.
• Original Assignee: Miroculus Inc.
• Inventors: Hong, Ik Pyo, Barbulovic-Nad, Irena, Soto-Moreno, Jorge Abraham
• Primary Examiner(s): Noguerola, Alexander S
• Application Number:
  US16/324,420
• Publication Number:
  US 20190217301A1
• Time in Patent Office: 945 Days
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension

Abstract

Digital microfluidics apparatuses (e.g., devices and systems) configured to determine provide feedback on the location, rate of movement, rate of evaporation and/or size (or other physical characteristic) of one or more, and preferably more than one, droplet in the gap region of a digital microfluidics (DMF) apparatus.

Claims

1. A digital microfluidic (DMF) apparatus with parallel droplet detection, the apparatus comprising:

a first plate having a plurality of actuation electrodes;

a second plate having one or more ground electrodes, wherein the second plate is spaced opposite from the first plate by a gap;

a voltage source;

a plurality of sensing circuits, wherein a sensing circuit from the plurality of sensing circuits is electrically connected to each actuation electrode, wherein each sensing circuit is configured to detect a charge voltage of a capacitor in a charging circuit of the sensing circuit, further wherein each sensing circuit of the plurality of sensing circuits comprises the charging circuit, a discharging circuit, and an analog-to-digital converter; and

a controller configured to alternate between applying voltage from the voltage source to the first plate and the second plate, wherein applying voltage to the first plate comprises applying voltage to one or more actuation electrodes from the plurality of actuation electrodes to move one or more droplets within the gap, and wherein applying voltage to the second plate comprises applying voltage to the one or more ground electrodes, further wherein the controller is configured to sense, in parallel, the location of one or more droplets relative to the plurality of actuation electrodes based on input from each of the sensing circuits when applying voltage to the second plate.

2. The apparatus of claim 1, wherein the discharging circuit comprises a transistor and a ground.

3. The apparatus of claim 1, wherein the charging circuit comprises a capacitor and a diode.

4. The apparatus of claim 1, wherein the ADC is configured to detect the charged voltage of the charging circuit.

5. The apparatus of claim 1, wherein the controller is configured to sequentially activate the discharge circuit, then the charging circuit, and to receive the charged voltage of the charging circuit from the ADC in parallel for all of the sensing circuits of the plurality of sensing circuits.

6. The apparatus of claim 1, further comprising a forward/reverse switch connected between the voltage source, the one or more ground electrodes, and the plurality of actuation electrodes, wherein the controller is configured to operate the forward/reverse switch to switch between applying voltage to the first plate and the second plate.

7. The apparatus of claim 1, further comprising a plurality of electrode switches, wherein each electrode switch from the plurality of electrode switches is connected to an actuation electrode of the plurality of actuation electrodes and is controlled by the controller through a switch controller to apply voltage from the voltage source to the actuation electrode.

8. The apparatus of claim 1, wherein the voltage supply comprises a high-voltage supply.

9. The apparatus of claim 1, wherein the controller is configured to compare a voltage sensed by each of the plurality of sensing circuits to a threshold voltage value to determine the location of one or more droplets relative to the plurality of actuation electrodes.

10. The apparatus of claim 1, wherein the controller is configured to compare a voltage sensed by each of the plurality of sensing circuits to a predetermined voltage value or range of voltage values to determine the size of one or more droplets.

11. A method of simultaneously determining the locations of multiple drops in a digital microfluidics (DMF) apparatus, the method comprising:

applying voltage to a plurality of actuation electrodes in a first plate to move one or more droplets within a gap between the first plate and a second plate;

applying voltage to one or more ground electrodes in the second plate;

concurrently sensing, in a plurality of sensing circuits, wherein each actuation electrode is associated with a separate sensing circuit from the plurality of sensing circuits, a charging voltage while applying voltage to the one or more ground electrodes; and

determining a location of the one or more droplets relative to the plurality of actuation electrodes based on the sensed charging voltages by comparing the sensed charging voltages to a predetermined value or range of values to determine if a droplet is on or adjacent to an actuation electrode.

12. The method of claim 11, wherein applying voltage to the plurality of actuation electrodes and applying voltage to the one or more ground electrodes comprises applying voltage from the same high voltage source.

13. The method of claim 11, wherein applying voltage to the plurality of actuation electrodes comprises sequentially applying voltage to adjacent actuation electrodes.

14. The method of claim 11 further comprising re-applying voltage to one or more of the plurality of actuation electrodes based on the determined location of the one or more droplets.

15. The method of claim 11, wherein applying voltage to one or more ground electrodes in the second plate comprises applying voltage to the one or more ground electrodes without applying voltage to the actuation electrodes in the first plate.

16. The method of claim 11, further comprising discharging voltage in each of the sensing circuits in the first plate prior to applying voltage to the one or more ground electrodes.

17. The method of claim 11, further comprising charging a capacitor in each of the sensing circuits of a plurality of sensing circuits in the first plate when applying voltage to the one or more ground electrodes.

18. The method of claim 11, further comprising discharging voltage in each of the sensing circuits prior to applying voltage to the one or more ground electrodes and then charging a capacitor in each of the sensing circuits in the plurality of sensing circuits when applying voltage to the one or more ground electrodes.

19. The method of claim 11, further comprising determining the size of the one or more droplets based on the sensed charging voltages.

20. The method of claim 11, further comprising correcting droplet motion based on the determined location of the one or more droplets.

21. The method of claim 11, further comprising determining an evaporation rate based on the sensed charging voltages.

22. A method of simultaneously determining size or the locations of multiple drops in a digital microfluidics (DMF) apparatus, the method comprising:

applying voltage to a plurality of actuation electrodes in a first plate to move one or more droplets within a gap between the first plate and a second plate;

discharging voltage in each sensing circuit of a plurality of sensing circuits when not applying voltage to the plurality of actuation electrodes in the first plate, wherein each actuation electrode is associated with a separate sensing circuit from the plurality of sensing circuits;

applying voltage to one or more ground electrodes in the second plate after discharging the voltage;

concurrently sensing, in each of the sensing circuits, a charging voltage while applying voltage to the one or more ground electrodes; and

determining a size or location of the one or more droplets relative to the plurality of actuation electrodes based on the sensed charging voltages.

23. A method of simultaneously determining the locations of multiple drops in a digital microfluidics (DMF) apparatus, the method comprising:

applying voltage to a plurality of actuation electrodes in a first plate to move one or more droplets within a gap between the first plate and a second plate;

applying voltage to one or more ground electrodes in the second plate;

concurrently sensing, in a plurality of sensing circuits, wherein each actuation electrode is associated with a separate sensing circuit from the plurality of sensing circuits, a charging voltage while applying voltage to the one or more ground electrodes;

determining a location of the one or more droplets relative to the plurality of actuation electrodes based on the sensed charging voltages; and

determining the size of the one or more droplets based on the sensed charging voltages.

24. A method of simultaneously determining the locations of multiple drops in a digital microfluidics (DMF) apparatus, the method comprising:

applying voltage to a plurality of actuation electrodes in a first plate to move one or more droplets within a gap between the first plate and a second plate;

applying voltage to one or more ground electrodes in the second plate;

concurrently sensing, in a plurality of sensing circuits, wherein each actuation electrode is associated with a separate sensing circuit from the plurality of sensing circuits, a charging voltage while applying voltage to the one or more ground electrodes;

determining a location of the one or more droplets relative to the plurality of actuation electrodes based on the sensed charging voltages; and

determining an evaporation rate based on the sensed charging voltages.