Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
First Claim
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1. A method for nucleic acid sequencing, comprising:
- disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array;
exposing the plurality of template polynucleotide strands to a series of flows of nucleotide species, the series comprising a combinatorial ordering of flows comprising a plurality of different adjacent 4-flow permutations of different nucleotide species A, C, G, and T, wherein adjacent 4-flow permutations in the combinatorial ordering of flows differ by a single transposition of two nucleotide species; and
obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the plurality of template polynucleotide strands.
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Abstract
A method for nucleic acid sequencing includes: disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array; exposing template polynucleotide strands to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
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Citations
5 Claims
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1. A method for nucleic acid sequencing, comprising:
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disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array; exposing the plurality of template polynucleotide strands to a series of flows of nucleotide species, the series comprising a combinatorial ordering of flows comprising a plurality of different adjacent 4-flow permutations of different nucleotide species A, C, G, and T, wherein adjacent 4-flow permutations in the combinatorial ordering of flows differ by a single transposition of two nucleotide species; and obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the plurality of template polynucleotide strands. - View Dependent Claims (2, 3, 4, 5)
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Specification