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Phase-protecting reagent flow orderings for use in sequencing-by-synthesis

  • US 10,597,711 B2
  • Filed: 04/09/2013
  • Issued: 03/24/2020
  • Est. Priority Date: 04/08/2011
  • Status: Active Grant
First Claim
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1. A method for nucleic acid sequencing, comprising:

  • disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array;

    exposing the plurality of template polynucleotide strands to a series of flows of nucleotide species, the series comprising a combinatorial ordering of flows comprising a plurality of different adjacent 4-flow permutations of different nucleotide species A, C, G, and T, wherein adjacent 4-flow permutations in the combinatorial ordering of flows differ by a single transposition of two nucleotide species; and

    obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the plurality of template polynucleotide strands.

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