Method of selecting analyte to samples using a lateral flow device
First Claim
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1. A lateral flow assay device for detecting the presence of an analyte within a test sample, the lateral flow assay device comprises:
- a chromatographic medium that includes;
a sample loading zone located upstream of a detection zone;
a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier capable of forming a complex with the analyte, and wherein said reporting carrier comprises a carrier and at least one proficient enzyme;
a detection zone, wherein the detection zone comprises a capture component for the analyte, and an indicator for indicating the presence or absence of a product from a reaction of the proficient enzyme and a substrate thereby indicating the presence or absence of the analyte; and
a control zone, wherein the control zone comprises a further capture component for the uncomplexed reporting carrier from the detection zone, and a further indicator for indicating the presence or absence of a product from a reaction of the proficient enzyme and the substrate thereby indicating the presence or absence of uncomplexed reporting carrier.
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Abstract
Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.
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69 Claims
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1. A lateral flow assay device for detecting the presence of an analyte within a test sample, the lateral flow assay device comprises:
a chromatographic medium that includes; a sample loading zone located upstream of a detection zone; a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier capable of forming a complex with the analyte, and wherein said reporting carrier comprises a carrier and at least one proficient enzyme; a detection zone, wherein the detection zone comprises a capture component for the analyte, and an indicator for indicating the presence or absence of a product from a reaction of the proficient enzyme and a substrate thereby indicating the presence or absence of the analyte; and a control zone, wherein the control zone comprises a further capture component for the uncomplexed reporting carrier from the detection zone, and a further indicator for indicating the presence or absence of a product from a reaction of the proficient enzyme and the substrate thereby indicating the presence or absence of uncomplexed reporting carrier. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
Specification