Engineered enzymes

• US 10,604,746 B1
• Filed: 10/21/2019
• Issued: 03/31/2020
• Est. Priority Date: 10/22/2018
• Status: Active Grant

• Current Assignee: Inscripta, Inc.
• Original Assignee: Inscripta, Inc.
• Inventors: Mijts, Benjamin, Kim, Juhan, Mir, Aamir, Seamon, Kyle, Garst, Andrew
• Primary Examiner(s): Saidha, Tekchand
• Application Number:
  US16/658,948
• Publication Number:
  US 20200123517A1
• Time in Patent Office: 162 Days
• Field of Search: 435194
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113

Abstract

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

Claims

1. An engineered nucleic acid-guided nuclease having a protospacer adjacent motif (PAM) preference different than the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1, wherein the engineered nucleic acid-guided nuclease has a sequence comprising any of SEQ ID Nos. 2, 3, 11, 12, 13, 14, 67, or 68.

2. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ. ID. No. 2.

3. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ. ID. No. 3.

4. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ. ID. No. 67.

5. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ. ID. No. 68.

6. A nucleic acid-guided nuclease cocktail comprising any one of the nucleic acid-guided nucleases of claim 1.

7. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 11.

8. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 12.

9. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 13.

10. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 14.