Focused libraries of genetic packages
First Claim
Patent Images
1. An antibody library comprising a first set of variegated DNA molecules encoding a first collection of antibody light chains (LC), wherein each light chain comprises an LC CDR1 region, an LC CDR2 region, and an LC CDR3 region, and wherein:
- (a) the first collection of antibody light chains are kappa light chains, which comprise a plurality of LCκ
CDR3 regions selected from the group consisting of;
(1) QQ<
3>
<
1>
<
1>
<
1>
P<
1>
T (SEQ ID NO;
16), wherein <
1>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; and
<
3>
is a mixture of amino acid residues Y, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, and W;
(2) QQ<
3>
<
3>
<
1>
<
1>
<
1>
P (SEQ ID NO;
103), wherein <
1> and
<
3>
are as defined in (1) above;
(3) QQ<
3>
<
2>
<
1>
<
1>
PP<
1>
T (SEQ ID NO;
17), wherein <
1> and
<
3>
are as defined in (1) above and <
2>
is a mixture of amino acid residues S, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, W, and Y; and
(4) a mixture of any of (1) to (3) set forth above;
or(b) the first collection of antibody light chains are lambda light chains, which comprise a plurality of LCλ
CDR3 regions selected from the group consisting of;
(1) <
4>
<
5>
<
4>
<
2>
<
4>
S<
4>
<
4>
<
4>
<
4>
V (SEQ ID NO;
106), wherein <
2>
is a mixture of amino acid residues D, N, A, E, F, G, H, I, K, L, M, P, Q, R, S, T, V, W, and Y;
<
4>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, and W; and
<
5>
is a mixture of amino acid residues S, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, W, and Y;
(2) <
5>
SY<
1>
<
5>
S<
5>
<
1>
<
4>
V (SEQ ID NO;
19), wherein <
1>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, and Y; and
<
4> and
<
5>
are as defined in (1) above; and
(3) a mixture of (1) and (2) set forth above.
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Abstract
Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.
-
Citations
16 Claims
-
1. An antibody library comprising a first set of variegated DNA molecules encoding a first collection of antibody light chains (LC), wherein each light chain comprises an LC CDR1 region, an LC CDR2 region, and an LC CDR3 region, and wherein:
-
(a) the first collection of antibody light chains are kappa light chains, which comprise a plurality of LCκ
CDR3 regions selected from the group consisting of;(1) QQ<
3>
<
1>
<
1>
<
1>
P<
1>
T (SEQ ID NO;
16), wherein <
1>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; and
<
3>
is a mixture of amino acid residues Y, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, and W;(2) QQ<
3>
<
3>
<
1>
<
1>
<
1>
P (SEQ ID NO;
103), wherein <
1> and
<
3>
are as defined in (1) above;(3) QQ<
3>
<
2>
<
1>
<
1>
PP<
1>
T (SEQ ID NO;
17), wherein <
1> and
<
3>
are as defined in (1) above and <
2>
is a mixture of amino acid residues S, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, W, and Y; and(4) a mixture of any of (1) to (3) set forth above;
or(b) the first collection of antibody light chains are lambda light chains, which comprise a plurality of LCλ
CDR3 regions selected from the group consisting of;(1) <
4>
<
5>
<
4>
<
2>
<
4>
S<
4>
<
4>
<
4>
<
4>
V (SEQ ID NO;
106), wherein <
2>
is a mixture of amino acid residues D, N, A, E, F, G, H, I, K, L, M, P, Q, R, S, T, V, W, and Y;
<
4>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, and W; and
<
5>
is a mixture of amino acid residues S, A, D, E, F, G, H, I, K, L, M, N, P, Q, R, T, V, W, and Y;(2) <
5>
SY<
1>
<
5>
S<
5>
<
1>
<
4>
V (SEQ ID NO;
19), wherein <
1>
is a mixture of amino acid residues A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, and Y; and
<
4> and
<
5>
are as defined in (1) above; and(3) a mixture of (1) and (2) set forth above. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
Specification