Biosensor, measuring instrument for biosensor, and method of quantifying substrate

• US 10,605,757 B2
• Filed: 12/05/2017
• Issued: 03/31/2020
• Est. Priority Date: 11/30/2000
• Status: Expired due to Fees

• Current Assignee: PHC Holdings Corporation
• Original Assignee: PHC Holdings Corporation
• Inventors: Miyazaki, Shoji, Tokunaga, Hiroyuki, Tokuno, Yoshinobu
• Primary Examiner(s): Kaur, Gurpreet
• Assistant Examiner(s): Rosenwald, Steven E
• Application Number:
  US15/831,642
• Publication Number:
  US 20180095047A1
• Time in Patent Office: 847 Days
• CPC Class Codes: A61B 2562/0295 Strip shaped analyte sensors for apparatus classified in A61B5/145 or A61B5/157
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A method of measuring a quantity of a substrate contained in sample liquid is provided. This method can reduce measurement errors caused by a biosensor. The biosensor includes at least a pair of electrodes on an insulating board and is inserted into a measuring device which includes a supporting section for supporting detachably the biosensor, plural connecting terminals to be coupled to the respective electrodes, and a driving power supply which applies a voltage to the respective electrodes, and a driving power terminals. One of the electrodes of the biosensor is connected to the first and second connecting terminals of the measuring device only when the biosensor is inserted into the measuring device in a given direction, and has a structure such that the electrode becomes conductive between the first and second connecting terminals due to a voltage application by the driving power supply.

Claims

1. A method of measuring a quantity of a substrate in a sample liquid by inserting a biosensor into a measuring device,

the biosensor comprising:

an insulating board;

an electrode part which includes a counter electrode, a measuring electrode and a detecting electrode disposed on at least a part of the insulating board;

a sample supplying path which supplies the sample liquid to the electrode part; and

a reagent layer at least partially disposed in the sample supplying path; and

the measuring device comprising;

a supporting section which detachably supports the biosensor;

a connecting terminal which applies a voltage to the electrode part; and

a driving power supply;

the method comprising;

applying the voltage to the electrode part by the driving power supply when the biosensor is inserted into the supporting section;

detecting a start of supplying the sample liquid to the sample supplying path based on a first output signal from the counter electrode and the measuring electrode of the electrode part;

measuring time from the start of supplying the sample liquid to a predetermined time, and

determining whether or not additional sample liquid is supplied to the sample supplying path from the start of supplying the sample liquid to the predetermined time based on a second output signal from the measuring electrode and the detecting electrode of the electrode part.

2. The method of claim 1, wherein the predetermined time is 10 seconds.

3. The method of claim 1, the method further comprising terminating a measurement of the quantity of the substrate if a sufficient quantity of the sample liquid has not been supplied when an elapsed time exceeds the predetermined time.