Device, system, and method including micro-patterned cell treatment array

• US 10,610,635 B2
• Filed: 07/11/2018
• Issued: 04/07/2020
• Est. Priority Date: 06/24/2011
• Status: Active Grant

• Current Assignee: Immunovalent Therapeutics, Inc.
• Original Assignee: Gearbox LLC (Intellectual Ventures LLC)
• Inventors: Hyde, Roderick A., Wood, Jr., Lowell L.
• Primary Examiner(s): Martinez, Rebecca L
• Application Number:
  US16/032,219
• Publication Number:
  US 20180326143A1
• Time in Patent Office: 636 Days
• Field of Search: None
• CPC Class Codes: A61B 17/435 for embryo or ova transplantation
• CPC Class Codes: A61M 1/3689 by biological cells
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: C12Q 3/00 Condition responsive control processes apparatus therefor C12M1/36; controlling or regulating in general G05
• CPC Class Codes: G01N 33/505 involving T-cells
• CPC Class Codes: G01N 33/5091 for testing the pathological state of an organism
• CPC Class Codes: Y10T 436/115831 Condition or time responsive

Abstract

Devices, systems, or methods are disclosed herein for treatment of disease in a vertebrate subject. The device can include a quasi-planar substrate; and one or more laterally-mobile effector molecule types at least partially embedded within the quasi-planar substrate, wherein the one or more laterally-mobile effector molecule types is configured to interact with one or more cell types. The device can further include one or more sensors configured to detect at least one aspect of an interaction between the at least one of the one or more laterally-mobile effector molecule types and the one or more cell types; and a controller in communication with the one or more sensors, wherein the controller is configured to responsively initiate modification of at least one of the one or more laterally-mobile effector molecule types, the quasi-planar substrate, and the one or more cell types.

Claims

1. A device, comprising:

a quasi-planar substrate;

one or more cell bays on the quasi-planar substrate, each cell bay including one or more fixed effector molecules and configured to interact with one or more cells;

at least one channel in fluid communication with the one or more cell bays and configured for providing passage of one or more cells proximate the one or more cell bays;

at least one gate or valve coupled to at least one of the one or more cell bays and configured to provide passage of the one or more cells to at least one cell trap in the one or more cell bays;

one or more sensors configured to detect at least one of binding or release from binding between the one or more fixed effector molecules and the one or more cells;

at least one controller in communication with the one or more sensors configured to control at least one of the gate or valve, or the fluid channel, in response to the sensed binding or release of one or more cells from the one or more fixed effector molecules.

2. The device of claim 1, wherein the one or more cells include at least one of a dendritic cell, stem cell, lymphocyte or NK cell.

3. The device of claim 2, wherein the stem cell includes at least one of a hematopoietic stem cell, myeloid stem cell, or pluripotent stem cell.

4. The device of claim 1, wherein the one or more cells include at least one of a Treg cell.

5. The device of claim 1, wherein the one or more fixed effector molecules include at least one of an antigen, major histocompatibility complex (MHC), antigen-loaded MHC, co-stimulatory molecule, or cytokine.

6. The device of claim 5, wherein the antigen includes at least one of a tumor-associated antigen, or viral peptide antigen.

7. The device of claim 1, wherein the one or more fixed effector molecules include at least one of an integral membrane protein, glycophosphatidylinositol-anchored protein, fusion protein, conjugated protein, aptamer or polymer.

8. The device of claim 1, wherein the one or more sensors include at least one of a thermal detector, an electrical detector, a chemical detector, an optical detector, an ion detector, a biological detector, a radioisotope detector, an electrochemical detector, a radiation detector, an acoustic detector, a magnetic detector, a capacitive detector, a pressure detector, an ultrasonic detector, an infrared detector, a microwave motion detector, a radar detector, an electric eye, an image sensor.

9. The device of claim 1, further including at least one micropump operably coupled to the controller.

10. The device of claim 9, wherein the at least one micropump is configured to increase the medium level in a cell bay to assist in movement of the cell out of the cell bay.

11. The device of claim 1, wherein the membrane structure includes at least one of a lipid bilayer or a lipid monolayer.