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Scaffolded nucleic acid polymer particles and methods of making and using

  • US 10,612,017 B2
  • Filed: 09/04/2018
  • Issued: 04/07/2020
  • Est. Priority Date: 05/29/2009
  • Status: Active Grant
First Claim
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1. A method for forming a particle, the method comprising:

  • suspending an emulsion in an initiator-saturated oil-based continuous phase, the emulsion comprising the continuous phase and a disperse phase, the disperse phase including an acrylamide monomer, a bis-acrylamide crosslinker, a surfactant, and an acrydite oligonucleotide in an aqueous solution;

    heating the emulsion to polymerize the acrylamide monomer, bis-acrylamide crosslinker and acrydite oligonucleotide to form acrylamide gel particles having a plurality of oligonucleotides attached through a polymer network of the acrylamide gel; and

    removing the initiator-saturated oil-based continuous phase; and

    resuspending the acrylamide gel particles in a buffered aqueous solution,wherein the acylamide gel particles have a coefficient of variance of volume of not greater than 15%, a total monomer percentage of the acrylamide gel particles is in a range of 3% to 20%, and the acrylamide gel particles are permeable to proteins having a size in the range of 50 kilodaltons to 200 kilodaltons.

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