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Selective oxidation of 5-methylcytosine by TET-family proteins

  • US 10,612,076 B2
  • Filed: 04/10/2017
  • Issued: 04/07/2020
  • Est. Priority Date: 09/26/2008
  • Status: Active Grant
First Claim
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1. A method comprising:

  • regulating a methylation status of one or more cytosine bases in an adult cell that is isolated and purified, wherein the regulating comprises contacting the adult cell with an effective amount of;

    (i) an enzyme or fragment thereof that converts;

    (a) 5-methylcytosine to 5-hydroxymethylcytosine,(b) 5-methylcytosine to an acid beyond hydroxymethlyation, or(c) 5-methylcytosine to an aldehyde beyond hydroxymethlyation;

    (ii) an inhibitor that decreases or prevents hydroxymethylation, wherein the inhibitor comprises siRNA or shRNA;

    or(iii) a combination thereof, andwherein the methylation status of the one or more cytosine bases in the adult cell is regulated.

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