Process for producing a PUFA-containing feedstuff by extruding a PUFA-containing biomass

• US 10,619,175 B2
• Filed: 09/22/2015
• Issued: 04/14/2020
• Est. Priority Date: 10/02/2014
• Status: Active Grant

• Current Assignee: Evonik Operations GmbH (Rag-Stiftung)
• Original Assignee: Evonik Operations GmbH (Rag-Stiftung)
• Inventors: Rabe, Christian, Silva, Amelia Claudia, Eils, Stefan, Priefert, Horst
• Primary Examiner(s): Ware, Deborah K
• Application Number:
  US15/516,044
• Publication Number:
  US 20170306365A1
• Time in Patent Office: 1,666 Days
• CPC Class Codes: A21D 2/165 Triglycerides
• CPC Class Codes: A23D 7/001 Spread compositions characterised by ingredients other than fatty acid triglycerides A23D7/0056
• CPC Class Codes: A23D 7/005 characterised by ingredients other than fatty acid triglycerides
• CPC Class Codes: A23D 7/0053 Compositions other than spreads
• CPC Class Codes: A23D 7/0056 Spread compositions
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/16 Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions A23K10/12 takes precedence
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 20/163 Sugars; Polysaccharides
• CPC Class Codes: A23K 40/25 by extrusion
• CPC Class Codes: A23K 40/30 by encapsulating; by coating
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A61K 31/20 having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11B 5/00 Preserving by using additives, e.g. anti-oxidants
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
• CPC Class Codes: Y02A 40/818 Alternative feeds for fish, e.g. in aquacultures

Abstract

In accordance with the invention, it was found, surprisingly, that a polyunsaturated fatty acids (PUFAs)-comprising biomass with other feedstuff components can be extruded at a low energy input of 12-28 Wh/kg to give an extrudate with a very high oil load capacity.

Claims

1. A feedstuff extrudate comprising a PUFA-comprising biomass, wherein the PUFA-comprising biomass comprises microbial cells of the taxon Labyrinthulomycetes, and has a sulphate content of 25 to 60 g/kg based on dry matter, and wherein the feedstuff extrudate has an oil load capacity of at least 0.35 g of oil per g of feedstuff extrudate.

2. The feedstuff extrudate of claim 1, wherein said biomass comprises a sulphate content 25 to 50 g/kg based on the dry matter.

3. The feedstuff extrudate of claim 1, wherein the PUFA-comprising biomass comprises microbial cells of the family Thraustochytriaceae.

4. The feedstuff extrudate of claim 1, wherein the PUFA-comprising biomass comprises microbial cells of the genera:

Thraustochytrium, Schizochytrium, Aurantiochytrium, Oblongichytrium or Ulkenia.

5. The feedstuff extrudate of claim 1, wherein the PUFA-comprising biomass comprises microbial cells of the species Aurantiochytrium limacinum.

6. The feedstuff extrudate of claim 1, wherein the biomass has a sulphate content of 25-35 g/kg based on the dry matter.

7. The feedstuff extrudate of claim 1, comprising:

a) a total protein content of 30 to 60% by weight;

b) a total fat content of 15 to 35% by weight;

c) a total starch content of not more than 25% by weight; and

d) a biomass content of 2 to 22% by weight.

8. The feedstuff extrudate of claim 7, further comprising one or more of:

e) a polyunsaturated fatty acid (PUFAs) content of 2 to 12% by weight;

f) an omega-3 fatty acid content of 1 to 6% by weight; and

g) a DHA content of 0.5 to 3% by weight.

9. The feedstuff extrudate of claim 1, comprising:

a) a total protein content of 40 to 50% by weight;

b) a total fat content of 22 to 28% by weight;

c) a total starch content of 7 to 13% by weight;

d) a Thraustochytriaceae biomass content of 10 to 16% by weight;

and further comprising one or more of the following;

e) a polyunsaturated fatty acid (PUFA) content of 5 to 8% by weight;

f) an omega-3 fatty acid content of 2.5 to 4% by weight; and

g) a DHA content of 1.2 to 2.0% by weight.

10. The feedstuff extrudate of claim 4, wherein the PUFA-comprising biomass has a sulphate content of 25 to 40 g/kg based on the dry matter.

11. The feedstuff extrudate of claim 10, further comprising:

a) a total protein content of 35 to 55% by weight;

b) a total fat content 18 to 32% by weight; and

c) a total starch content of 5 to 15% by weight.

12. The feedstuff extrudate of claim 11, further comprising:

d) a Thraustochytriaceae biomass content of 4 to 20% by weight;

e) a polyunsaturated fatty acid (PUFAs) content of 3 to 10% by weight;

f) an omega-3 fatty acid content of 2 to 4.5% by weight; and

g) a DHA content of 0.8 to 2.5% by weight.

13. A method of growing animals by feeding them a feedstuff extrudate comprising a PUFA-comprising biomass, wherein the PUFA-comprising biomass comprises microbial cells of the taxon Labyrinthulomycetes and has a sulphate content of 25 to 60 g/kg based on dry matter, and wherein the feedstuff extrudate has an oil load capacity of at least 0.35 g of oil per g of feedstuff extrudate.

14. The method of growing animals of claim 13, wherein the PUFA-comprising biomass comprises microbial cells of the family Thraustochytriaceae and has a sulphate content of 25 to 50 g/kg, based on the dry matter and wherein the feedstuff extrudate comprises:

a) a total protein content of 30 to 60% by weight;

b) a total fat content of 15 to 35% by weight;

c) a total starch content of not more than 25% by weight; and

d) a biomass content of 2 to 22% by weight.

15. The method of growing animals of claim 13, wherein the biomass comprises microbial cells of the species Aurantiochytrium limacinum and wherein the feedstuff extrudate further comprises one or more of the following:

e) a polyunsaturated fatty acid (PUFAs) content of 2 to 12% by weight;

f) an omega-3 fatty acid content of 1 to 6% by weight; and

g) a DHA content of 0.5 to 3% by weight.

16. The method of claim 14, wherein the PUFA-comprising biomass has a sulphate content of 25 to 40 g/kg based on the dry matter.

17. The method of claim 16, wherein the feedstuff extrudate further comprises:

a) a total protein content of 35 to 55% by weight;

b) a total fat content 18 to 32% by weight; and

c) a total starch content of 5 to 15% by weight.

18. The method of claim 17, wherein the feedstuff extrudate further comprises:

d) a Thraustochytriaceae biomass content, of 4 to 20% by weight;

e) a polyunsaturated fatty acid (PUFAs) content of 3 to 10% by weight;

f) an omega-3 fatty acid content of 2 to 4.5% by weight; and

g) a DHA content of 0.8 to 2.5% by weight.