Device and methods of using device for detection of hyperammonemia
First Claim
1. A method of quantifying a concentration of ammonia or ammonium ion in a sample comprising contacting a sample of bodily fluid to a biosensor, wherein the biosensor comprises:
- (i) at least a first and second vessel;
(ii) a fluid exchange opening positioned between the first and the second vessel;
(iii) at least one conduit in fluid communication with the at least first vessel, the at least one conduit configured to receive a fluid from a point external to the biosensor; and
(iv) a membrane positioned at the fluid exchange opening;
wherein the membrane comprises an ionomer;
wherein the at least one conduit is configured to hold a sample volume from about 5 μ
l to about 100 μ
l; and
wherein the first vessel or the second vessel comprises, individually or in combination;
a hypohalite, an alkali buffer, and at least one phenolic reagent or indophenol related compound;
wherein the sample is in contact with the biosensor for a sufficient period of time to allow modification of the phenolic reagent or indophenol related compound to become modified into a phenolic or indophenol reaction product.
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Accused Products
Abstract
The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perfluorinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.
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Citations
5 Claims
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1. A method of quantifying a concentration of ammonia or ammonium ion in a sample comprising contacting a sample of bodily fluid to a biosensor, wherein the biosensor comprises:
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(i) at least a first and second vessel; (ii) a fluid exchange opening positioned between the first and the second vessel; (iii) at least one conduit in fluid communication with the at least first vessel, the at least one conduit configured to receive a fluid from a point external to the biosensor; and (iv) a membrane positioned at the fluid exchange opening; wherein the membrane comprises an ionomer; wherein the at least one conduit is configured to hold a sample volume from about 5 μ
l to about 100 μ
l; andwherein the first vessel or the second vessel comprises, individually or in combination;
a hypohalite, an alkali buffer, and at least one phenolic reagent or indophenol related compound;wherein the sample is in contact with the biosensor for a sufficient period of time to allow modification of the phenolic reagent or indophenol related compound to become modified into a phenolic or indophenol reaction product. - View Dependent Claims (2, 3, 4, 5)
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Specification