Methods of decreasing feed conversion ratios in fowl

• US 10,624,366 B2
• Filed: 12/12/2018
• Issued: 04/21/2020
• Est. Priority Date: 04/15/2016
• Status: Active Grant

• Current Assignee: Native Microbials Incorporated
• Original Assignee: Ascus Biosciences, Inc.
• Inventors: Embree, Mallory, Gogul, Grant, Martino, Cameron, Pitt, Norm
• Primary Examiner(s): Devi, Sarvamangala
• Application Number:
  US16/218,074
• Publication Number:
  US 20190254311A1
• Time in Patent Office: 496 Days
• CPC Class Codes: A01K 45/00 Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 40/30 by encapsulating; by coating
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2200/3204 Probiotics, living bacteria to be ingested for action in the digestive tract
• CPC Class Codes: A61K 2035/115 Probiotics
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: C12R 2001/225 Lactobacillus

Abstract

The disclosure relates to methods for decreasing feed conversion ratios in fowl through administering isolated microorganisms to the fowl. In particular aspects, the disclosure provides methods of decreasing feed conversion ratios in fowl through administration of a Bacillus sp. to the fowl. The disclosure further relates to isolated microorganisms and compositions comprising the same.

Claims

1. A method for decreasing feed conversion ratio of a fowl, the method comprising:

(a) administering to the fowl an effective amount of a probiotic fowl supplement comprising;

a Bacillus sp. comprising a 16S nucleic acid sequence comprising SEQ ID NO;

13, and

(ii) a carrier suitable for fowl administration;

wherein the fowl administered the effective amount of the probiotic fowl supplement exhibits a decrease in feed conversion ratio, as compared to fowl not having been administered the supplement.

2. The method of claim 1, wherein the administering of the probiotic fowl supplement is an oral administration.

3. The method of claim 2, wherein the oral administration comprises combining the probiotic fowl supplement with the fowl'"'"'s feed, water, litter, medicine, or vaccine;

or spraying the fowl with the probiotic fowl supplement.

4. The method of claim 1, wherein the Bacillus sp. is in spore form.

5. The method of claim 1, wherein the carrier is selected from calcium carbonate, a mineral mixture, edible feed grade material, glycol, molasses, grease, tallow, soybean oil, rice bran oil, olive oil, corn oil, sesame oil, vegetable oil, and corn oil.

6. The method of claim 1, wherein the probiotic fowl supplement is administered to the fowl at least once a day.

7. The method of claim 6, wherein each administration of the probiotic fowl supplement comprises at least 10³ colony forming units of the Bacillus sp.

8. The method of claim 1, wherein the fowl is a broiler.

9. The method of claim 1, wherein the probiotic fowl supplement is dried.

10. The method of claim 9, wherein the Bacillus sp. is freeze dried.

11. The method of claim 9, wherein the probiotic fowl supplement has a moisture content of less than 10%.

12. The method of claim 1, wherein the probiotic fowl supplement is formulated as a water additive, a feed additive, a pre-pelleted feed additive, a pelleted feed additive, a post-pelleting-applied feed additive, a consumable liquid, a consumable solid, a consumable gel, or a gavage.

13. The method of claim 12, wherein the probiotic fowl supplement occurs within the pelleted feed.

14. The method of claim 1, wherein the feed conversion ratio of the fowl decreases by at least 1%.

15. The method of claim 14, wherein the feed conversion ratio of the fowl decreases by at least 5%.

16. The method of claim 1, wherein the administration of the probiotic fowl supplement shifts the gut microbiome of the fowl.

17. The method of claim 16, wherein the gut microbiome shift is a decrease in the relative abundance of one or more microbes that were present in the gut prior to administration of the probiotic fowl supplement.

18. The method of claim 16, wherein the gut microbiome shift is an increase in the relative abundance of one or more microbes that were present in the gut prior to administration of the probiotic fowl supplement.

19. The method of claim 18, wherein the gut microbiome shift is an increase in the relative abundance of obligate anaerobes.

20. The method of claim 18, wherein the gut microbiome shift is an increase in the relative abundance of lactic acid-producing microbes.

21. The method of claim 1, wherein the Bacillus sp. is deposited as NRRL B-67266.