Recombinant polymerases for incorporation of protein shield nucleotide analogs
First Claim
1. A composition comprising:
- a recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO;
1 or at least 80% identical to SEQ ID NO;
2, and which recombinant polymerase comprises a mutation selected from the group consisting of a T434A substitution, a T434S substitution and a T434V substitution, wherein identification of positions is relative to SEQ ID NO;
1.
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Accused Products
Abstract
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
77 Citations
21 Claims
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1. A composition comprising:
- a recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO;
1 or at least 80% identical to SEQ ID NO;
2, and which recombinant polymerase comprises a mutation selected from the group consisting of a T434A substitution, a T434S substitution and a T434V substitution, wherein identification of positions is relative to SEQ ID NO;
1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
- a recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO;
Specification