Free-solution response function interferometry
First Claim
1. A free-solution analytical method comprising detecting molecular interactions between a first non-immobilized analyte and a second non-immobilized analyte, wherein the detection is performed by refractive index sensing other than backscattering interferometry, or by circular dichroism, wherein at least one of the analytes is present in an amount of less than about 1.0×
- 10−
6 M.
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Abstract
Disclosed are methods for the free solution measurement of molecular interactions by refractive index sensing other than backscattering interferometry. The disclosed methods can have very low detection limits and/or very low sample volume requirements. Also disclosed are various biosensor applications of the disclosed techniques. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
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Citations
26 Claims
- 1. A free-solution analytical method comprising detecting molecular interactions between a first non-immobilized analyte and a second non-immobilized analyte, wherein the detection is performed by refractive index sensing other than backscattering interferometry, or by circular dichroism, wherein at least one of the analytes is present in an amount of less than about 1.0×
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8. A free-solution analytical method comprising the steps of:
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(a) providing a refractive index sensor for reception of a fluid sample to be analyzed; (b) introducing a first sample comprising a first non-immobilized analyte to be analyzed and a second sample comprising a second non-immobilized analyte to be analyzed onto the sensor, wherein the first analyte is allowed to interact with the second analyte; (c) interrogating the fluid sample with light; (d) detecting the light after interaction with the fluid sample, wherein the detected light is not backscattered; and (e) detecting a molecular interaction between the first and second analyte wherein at least one of the analytes is present in an amount of less than about 1.0×
10−
6 M. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 23, 24, 25)
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- 19. A free-solution analytical method comprising detecting a molecular change in an analyte, wherein the detection is performed by refractive index sensing other than backscattering interferometry, wherein detecting a molecular change comprises determining the degree of polymerization, protein folding, protein aggregation, blood oxygenation, the conformational state of an ion channel or membrane protein, or the hydration state of an ion channel or membrane protein, and wherein the analyte is present in an amount of less than about 1.0×
Specification