Systems and methods for multi-analysis
First Claim
Patent Images
1. A method of performing three or more assays for detecting a plurality of analytes in a biological sample from a subject, comprising:
- providing a cartridge in a refrigerated condition at a temperature less than room temperature,receiving the biological sample and the cartridge in a biological sample processing system, wherein;
a) the cartridge comprises;
a plurality of components including sample vessels, reagent units, and at least three assay units for performing the three or more assays to detect a plurality of analytes in the biological sample; and
b) the biological sample processing system comprises;
a sample handling system including a pipette; and
at least two detection units;
heating at least one portion of the cartridge using a movable heater on the biological sample processing system, wherein said heating comprises moving said movable heater from a first position to a second position wherein in the second position, at least a portion of the cartridge is inside the heater to pre-heat reagent units or assay units that are in the cartridge when the cartridge is in the biological sample processing system;
using said sample handling system for aliquoting at least a diluted portion of the sample into one of the plurality of said components while the cartridge remains inside the biological sample processing system;
decoupling one or more of said plurality of components from the cartridge while the cartridge remains inside the biological sample processing system to form one or more decoupled components;
performing in the biological sample processing system the three or more assays, wherein the three or more assays are performed at least partially in parallel and comprises leaving one or more of the one or more decoupled components of the cartridge at a location in the device separate from the cartridge;
detecting the plurality of analytes with at least one of the at least two detection unit in the biological sample processing system; and
after detecting the plurality of analytes, returning any decoupled components back to the cartridge while the cartridge remains inside the biological sample processing system;
wherein each of the reagent units is a container, a tube, a pipette tip, or a vessel;
wherein each of the assay units is a cuvette, a container, a tube, a pipette tip, or a vessel.
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Abstract
Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
630 Citations
20 Claims
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1. A method of performing three or more assays for detecting a plurality of analytes in a biological sample from a subject, comprising:
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providing a cartridge in a refrigerated condition at a temperature less than room temperature, receiving the biological sample and the cartridge in a biological sample processing system, wherein; a) the cartridge comprises; a plurality of components including sample vessels, reagent units, and at least three assay units for performing the three or more assays to detect a plurality of analytes in the biological sample; and b) the biological sample processing system comprises; a sample handling system including a pipette; and at least two detection units; heating at least one portion of the cartridge using a movable heater on the biological sample processing system, wherein said heating comprises moving said movable heater from a first position to a second position wherein in the second position, at least a portion of the cartridge is inside the heater to pre-heat reagent units or assay units that are in the cartridge when the cartridge is in the biological sample processing system; using said sample handling system for aliquoting at least a diluted portion of the sample into one of the plurality of said components while the cartridge remains inside the biological sample processing system; decoupling one or more of said plurality of components from the cartridge while the cartridge remains inside the biological sample processing system to form one or more decoupled components; performing in the biological sample processing system the three or more assays, wherein the three or more assays are performed at least partially in parallel and comprises leaving one or more of the one or more decoupled components of the cartridge at a location in the device separate from the cartridge; detecting the plurality of analytes with at least one of the at least two detection unit in the biological sample processing system; and after detecting the plurality of analytes, returning any decoupled components back to the cartridge while the cartridge remains inside the biological sample processing system; wherein each of the reagent units is a container, a tube, a pipette tip, or a vessel; wherein each of the assay units is a cuvette, a container, a tube, a pipette tip, or a vessel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of performing three or more assays for detecting a plurality of analytes in a biological sample from a subject, comprising:
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providing a cartridge in a refrigerated condition at a temperature less than room temperature, receiving the biological sample and the cartridge in a biological sample processing system, wherein; b) the cartridge comprises; a plurality of components including sample vessels, reagent units, and at least three assay units for performing the three or more assays to detect a plurality of analytes in the biological sample; and b) the biological sample processing system comprises; a sample handling system including a pipette; and at least two detection units; heating at least one portion of the cartridge using a movable heater on the biological sample processing system, wherein said heating comprises moving said movable heater from a first position to a second position wherein in the second position, at least a portion of the cartridge is inside the heater to pre-heat reagent units or assay units that are in the cartridge when the cartridge is in the biological sample processing system; using said sample handling system for aliquoting at least a diluted portion of the sample into one of the plurality of said components while the cartridge remains inside the biological sample processing system; decoupling one or more of said plurality of components from the cartridge while the cartridge remains inside the biological sample processing system to form one or more decoupled components; performing in the biological sample processing system the three or more assays, wherein the three or more assays are performed at least partially in parallel and comprises leaving one or more of the one or more decoupled components of the cartridge at a location in the device separate from the cartridge; detecting the plurality of analytes with at least one of the at least two detection unit in the biological sample processing system; and after detecting the plurality of analytes, returning any decoupled components back to the cartridge while the cartridge remains inside the biological sample processing system; wherein each of the reagent units is a container, a tube, a pipette tip, or a vessel; wherein each of the assay units is a cuvette, a container, a tube, a pipette tip, or a vessel; where the cartridge in the refrigerated condition has temperature in the range of about 2°
C. to about 8°
C.
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20. A method of performing three or more assays for detecting a plurality of analytes in a biological sample from a subject, comprising:
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providing a cartridge in a refrigerated condition at a temperature less than room temperature, receiving the biological sample and the cartridge in a biological sample processing system, wherein; c) the cartridge comprises; a plurality of components including sample vessels, reagent units, and at least three assay units for performing the three or more assays to detect a plurality of analytes in the biological sample; and b) the biological sample processing system comprises; a sample handling system including a pipette; and at least two detection units; heating at least one portion of the cartridge using a movable heater on the biological sample processing system, wherein said heating comprises moving said movable heater from a first position to a second position wherein in the second position, at least a portion of the cartridge is inside the heater to pre-heat reagent units or assay units that are in the cartridge when the cartridge is in the biological sample processing system; using said sample handling system for aliquoting at least a diluted portion of the sample into one of the plurality of said components while the cartridge remains inside the biological sample processing system; decoupling one or more of said plurality of components from the cartridge while the cartridge remains inside the biological sample processing system to form one or more decoupled components; performing in the biological sample processing system the three or more assays, wherein the three or more assays are performed at least partially in parallel and comprises leaving one or more of the one or more decoupled components of the cartridge at a location in the device separate from the cartridge; detecting the plurality of analytes with at least one of the at least two detection unit in the biological sample processing system; and after detecting the plurality of analytes, returning any decoupled components back to the cartridge while the cartridge remains inside the biological sample processing system; wherein each of the reagent units is a container, a tube, a pipette tip, or a vessel; wherein each of the assay units is a cuvette, a container, a tube, a pipette tip, or a vessel; wherein the heater is mounted below a cartridge receiving location in the device.
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Specification