Microbial compositions and methods of use for improving fowl production

• US 10,632,157 B2
• Filed: 07/23/2018
• Issued: 04/28/2020
• Est. Priority Date: 04/15/2016
• Status: Active Grant

• Current Assignee: Native Microbials Incorporated
• Original Assignee: Ascus Biosciences, Inc.
• Inventors: Embree, Mallory, Gogul, Grant, Martino, Cameron, Pitt, Norm
• Primary Examiner(s): Devi, Sarvamangala
• Application Number:
  US16/042,369
• Publication Number:
  US 20180333443A1
• Time in Patent Office: 645 Days
• CPC Class Codes: A23K 10/16 Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions A23K10/12 takes precedence
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A61K 2035/115 Probiotics
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/0056 Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: C12R 2001/07 Bacillus
• CPC Class Codes: C12R 2001/225 Lactobacillus

Abstract

The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial ensembles, and compositions comprising the same, in methods for modulating the production of poultry, disease resistance, and egg yield. In particular aspects, the disclosure provides methods of increasing feed efficiency, and methods of preventing colonization of pathogenic microbes.

Claims

1. A method of inhibiting colonization of or inhibiting prevalence and/or abundance of the fowl pathogen Clostridium perfringens in the gastrointestinal tract of a fowl, the method comprising orally administering to the fowl an effective amount of a microbial composition comprising:

(a) a purified population of one or more bacteria of the Bacillus genus having a 16S nucleic acid sequence that comprises SEQ ID NO;

13 and

(b) a carrier suitable for fowl administration,

wherein the purified population is present in the composition in an amount effective to inhibit the colonization of, the prevalence and/or the abundance of the fowl pathogen Clostridium perfringens in the gastrointestinal tract of the fowl as compared to a fowl not having been administered the composition.

2. The method of claim 1, wherein the fowl is a broiler chicken.

3. The method of claim 1, wherein the administering comprises feeding the microbial composition to the fowl.

4. The method of claim 1, wherein the one or more bacteria have a MIC score of at least about 0.1.

5. The method of claim 1, wherein the microbial composition is formulated as an encapsulation, tablet, capsule, pill, feed additive, food ingredient, food preparation, food supplement, water additive, water-mixed additive, heat-stabilized additive, moisture-stabilized additive, pre-pelleted feed additive, pelleted feed additive, post-pelleting applied feed additive, consumable solution, consumable spray additive, consumable, solid, consumable gel, or bolus.

6. The method of claim 1, wherein the method improved feed conversion, increased weight gain, and lowered mortality due to Clostridium perfringens in the fowl as compared to a fowl not having been administered the composition.

7. The method of claim 1, wherein the method decreased incidence of Clostridium perfringens-associated lesion formation in the fowl as compared to a fowl not having been administered the composition.