Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
First Claim
1. A composition including:
- a substrate;
a first polynucleotide coupled to the substrate;
a second polynucleotide hybridized to the first polynucleotide;
a catalyst coupled to a first nucleotide at the 3′
end of the second polynucleotide, wherein the catalyst is operable to cause a chemiluminogenic molecule to emit a photon, wherein the first nucleotide is coupled to a first moiety, and the catalyst is coupled to a second moiety, wherein the first nucleotide and the catalyst are coupled via the first and second moieties; and
a polymerase capable of extending the second polynucleotide hybridized to the first polynucleotide.
1 Assignment
0 Petitions
Accused Products
Abstract
Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.
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Citations
18 Claims
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1. A composition including:
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a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; a catalyst coupled to a first nucleotide at the 3′
end of the second polynucleotide, wherein the catalyst is operable to cause a chemiluminogenic molecule to emit a photon, wherein the first nucleotide is coupled to a first moiety, and the catalyst is coupled to a second moiety, wherein the first nucleotide and the catalyst are coupled via the first and second moieties; anda polymerase capable of extending the second polynucleotide hybridized to the first polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification