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Systems and methods for nucleic acid sequencing

  • US 10,633,701 B2
  • Filed: 08/07/2017
  • Issued: 04/28/2020
  • Est. Priority Date: 12/21/2007
  • Status: Active Grant
First Claim
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1. A method, comprising:

  • providing a first microfluidic droplet containing a nucleic acid probe and at least three distinguishable identification elements, wherein each of the at least three distinguishable identification elements is associated with a different oligonucleotide sequence, wherein each of the different oligonucleotide sequences differs by one nucleic acid residue;

    providing a second microfluidic droplet comprising a target nucleic acid; and

    fusing at least some of the fluid in the first fluidic droplet and at least some of the fluid in the second microfluidic droplet to form a fused droplet.

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