Miniaturized microscope for phase contrast and multicolor fluorescence imaging
First Claim
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1. A microscope, comprising:
- an illumination source configured to provide illumination beams to image a portion of a biological sample;
an optical unit configured to enable both phase contrast imaging and multicolor fluorescence imaging of the portion of the biological sample utilizing parallel point scanning; and
a detector configured to simultaneously acquire a plurality of point images at different locations of the portion of the biological sample.
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Abstract
A microscope is provided. The microscope includes an illumination source configured to provide illumination beams to image a portion of a biological sample. The microscope also includes an optical unit configured to enable both phase contrast imaging and multicolor fluorescence imaging of the portion of the biological sample utilizing parallel point scanning. The microscope further includes a detector configured to simultaneously acquire multiple point images at different locations of the portion of the biological sample.
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Citations
20 Claims
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1. A microscope, comprising:
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an illumination source configured to provide illumination beams to image a portion of a biological sample; an optical unit configured to enable both phase contrast imaging and multicolor fluorescence imaging of the portion of the biological sample utilizing parallel point scanning; and a detector configured to simultaneously acquire a plurality of point images at different locations of the portion of the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A microscope, comprising:
an optical unit configured to enable both phase contrast imaging and multicolor fluorescence imaging of a portion of a biological sample utilizing parallel point scanning, wherein the optical unit comprises; a first microlens array; an edge long-pass filter disposed on a backside of the first microlens array, wherein the edge long-pass filter is configured to form a respective condenser annulus associated with a respective lens element of the first microlens array, wherein the edge long-pass filter is configured to enable a portion of a beam at a first wavelength to pass through to the first microlens array, and the edge-long filter is transparent to beams at a second and a third wavelength different from the first wavelength; a second microlens array, wherein the second microlens array is configured to collimate a diffracted transmitted beam at the first wavelength from the biological sample, and the second microlens array is configured to collect fluorescent beams emitted by the biological sample at the second wavelength and the third wavelength; an absorption filter and an interference notch filter located downstream of the first and second microlens array, wherein the absorption filter and the interference notch filter are configured to be transparent to the beam at the first wavelength and block excitation beams of different wavelengths while only allowing emitted fluorescent beams at the second and third wavelengths to pass; and a third microlens array and a fourth microlens array located downstream of the absorption filter and the interference notch filter, and a pinhole array disposed between the third and fourth microlens array, and wherein the third microlens array, the pinhole array, and the fourth microlens array are configured to spatially filter the transmitted beam at the first wavelength and the undiffracted beam at the first wavelength that has passed through the biological sample and to spatially filter the emitted fluorescent beams at the second and third wavelengths; wherein the first, second, third, and fourth microlens arrays are focally aligned. - View Dependent Claims (15, 16, 17)
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18. A method of manufacturing an optical unit for a microscope configured to enable both phase contrast imaging and multicolor fluorescence imaging of a portion of a biological sample utilizing parallel point scanning, comprising:
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providing a first microlens array; disposing an edge long-pass filter on a backside of the first microlens array, wherein the edge long-pass filter is configured to form a respective condenser annulus associated with a respective lens element of the first microlens array, wherein the edge long-pass filter is configured to enable a portion of a beam at a first wavelength to pass through to the first microlens array, and the edge-long filter is transparent to beams at a second and a third wavelength different from the first wavelength; optically aligning a second microlens array with the first microlens array, wherein the second microlens array is configured to collimate a diffracted transmitted beam at the first wavelength from the biological sample, and the second microlens array is configured to collect fluorescent beams emitted by the biological sample at the second wavelength and the third wavelength; disposing an absorption filter and an interference notch filter downstream of the first and second microlens array, wherein the absorption filter and the interference notch filter are configured to be transparent to the beam at the first wavelength and block excitation beams of different wavelengths while only allowing emitted fluorescent beams at the second and third wavelengths to pass; and optically aligning a third microlens array, a fourth microlens array, and a pinhole array disposed between the third and fourth microlens array with the first and second microlens arrays downstream of the absorption filter and the interference notch filter, wherein the third microlens array, the pinhole array, and the fourth microlens array are configured to spatially filter the transmitted beam at the first wavelength and the undiffracted beam at the first wavelength that has passed through the biological sample and to spatially filter the emitted fluorescent beams at the second and third wavelengths. - View Dependent Claims (19, 20)
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Specification