Lettuce plants comprising resistance against Nasonovia ribisnigri biotype 1

• US 10,638,689 B2
• Filed: 10/29/2015
• Issued: 05/05/2020
• Est. Priority Date: 10/30/2014
• Status: Active Grant

• Current Assignee: Nunhems BV (BASF SE)
• Original Assignee: Nunhems BV (BASF SE)
• Inventors: Schaareman, Robert Theodorus Gerardus, Thomas, Vincent Pierre Andre, Van Der Arend, Adrianus J. M., Raedts, Robert Johannes Martinus, Julian Rodriguez, Olga
• Primary Examiner(s): Buran, Ashley K
• Assistant Examiner(s): Zhong, Wayne
• Application Number:
  US15/523,219
• Publication Number:
  US 20170318770A1
• Time in Patent Office: 1,650 Days
• Field of Search: None
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 5/12 Leaves
• CPC Class Codes: A01H 6/1472 Lactuca sativa [lettuce]
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8279 for biotic stress resistance, pathogen resistance, disease resistance
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism

Abstract

The present invention relates to the field of lettuce breeding, in particular to Quantitative Trait Loci for resistance against the lettuce aphid Nasonovia ribisnigri biotype Nr:1 and to cultivated lettuce comprising one or more of these Quantitative Trait Loci.

Claims

1. A Lactuca sativa plant comprising an introgression fragment from Lactuca virosa on chromosome 6 and/or on chromosome 7 which comprises a Quantitative Trait Locus (QTL) that confers resistance against Nasonovia ribisnigri biotype 1 (Nr:

1), and wherein said introgression fragment on chromosome 6 comprises QTL6.1 in the region starting at 77 Mb and ending at 162 Mb of chromosome 6 and wherein said introgression fragment on chromosome 7 comprises QTL7.1 in the region starting at 203 Mb and ending at 219 Mb of chromosome 7,

wherein said introgression fragment on chromosome 6 comprises at least 1 of the following markers;

a) the CC or CT genotype for the Single Nucleotide Polymorphism marker SNP1.23 in SEQ ID NO;

23;

b) the CC or CT genotype for the Single Nucleotide Polymorphism marker SNP 02 in SEQ ID NO;

2;

c) the TT or CT genotype for the Single Nucleotide Polymorphism marker SNP2.24 in SEQ ID NO;

24; and

/or

d) the AA or AC genotype for the Single Nucleotide Polymorphism marker SNP 03 in SEQ ID NO;

3, and

wherein the introgression fragment on chromosome 7 comprises at least 1 of the following markers;

e) the TT or TC genotype for the Single Nucleotide Polymorphism marker SNP 17 in SEQ ID NO;

17;

f) the TT or TC genotype for the Single Nucleotide Polymorphism marker SNP17.25 in SEQ ID NO;

25;

g) the GG or GC genotype for the Single Nucleotide Polymorphism marker SNP 18 in SEQ ID NO;

18; and

/or

h) the GG or GA genotype for the Single Nucleotide Polymorphism marker SNP 19 in SEQ ID NO;

19.

2. The plant according to claim 1, comprising both the introgression fragment on chromosome 6 and on chromosome 7.

3. The plant according to claim 1, wherein said introgression fragment on chromosome 6 further comprises:

i) the GG or GT genotype for the Single Nucleotide Polymorphism marker VSP1 in SEQ ID NO;

26;

or

j) the AA or AC genotype for the Single Nucleotide Polymorphism marker VSP3 in SEQ ID NO;

27.

4. The plant according to claim 1, wherein said introgression fragment on chromosome 7 further comprises:

k) the CC or CA genotype for the Single Nucleotide Polymorphism marker VSP2 in SEQ ID NO;

28, or

l) the GG or GA genotype for the Single Nucleotide Polymorphism marker VSP4 in SEQ ID NO;

29.

5. The plant according to claim 1, wherein the Lactuca sativa plant is loose-leaf lettuce, Romaine lettuce, Crisphead lettuce, Butterhead lettuce, Batavia lettuce or Stem lettuce.

6. The plant according to claim 1, wherein said introgression fragment is a fragment of chromosome 6 and/or 7 as found in L. virosa accession NCIMB42086.

7. A seed from which a plant according to claim 1 can be grown.

8. Leaves or heads of the plant according to claim 1.

9. A progeny plant of the lettuce plant according to claim 1, wherein said progeny plant retains the introgression fragment on chromosome 6 comprising QTL6.1 and/or chromosome 7 comprising QTL7.1.

10. The progeny plant according to claim 9, wherein the progeny plant is produced by one or more of:

selfing, crossing, mutation, double haploid production or transformation.

11. A part of a lettuce plant according to claim 1, wherein the part is selected from the group consisting of:

stems, cuttings, petioles, cotyledons, flowers, anthers, pollen, ovaries, roots, root tips, protoplasts, callus, microspores, stalks, ovules, shoots, seeds, embryos, embryo sacs, cells, buds, leaves, and meristems, and wherein said part comprises the introgression fragment on chromosome 6 comprising QTL6.1 and/or chromosome 7 comprising QTL7.1.

12. The plant according to claim 1, wherein said introgression fragment on chromosome 6 comprises the CC or CT genotype for the Single Nucleotide Polymorphism marker SNP_02 in SEQ ID NO:

2, and the AA or AC genotype for the Single Nucleotide Polymorphism marker for VSP3 in SEQ ID NO;

27.

13. The plant according to claim 1, wherein said introgression fragment on chromosome 7 comprises the GG or GC genotype for the Single Nucleotide Polymorphism marker for SNP_18 in SEQ ID 18, and the GG or GA genotype for the Single Nucleotide Polymorphism marker for VSP4 in SEQ ID NO:

29.