Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
First Claim
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1. A method for identifying an agent that affects a desired cellular response, the method comprising:
- a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, express oct4 and/or telomerase and are not tumorigenic, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent;
b) determining whether said agent affects said cellular response in said cells of the population.
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Abstract
The present invention relates generally to mammalian multipotent adult stem cells (MASC), and more specifically to methods for obtaining, maintaining and differentiating MASC to cells of multiple tissue types. Uses of MASC in the therapeutic treatment of disease are also provided.
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Citations
20 Claims
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1. A method for identifying an agent that affects a desired cellular response, the method comprising:
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a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, express oct4 and/or telomerase and are not tumorigenic, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent; b) determining whether said agent affects said cellular response in said cells of the population. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 20)
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12. A method for identifying an agent that affects a desired cellular response, the method comprising:
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a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, are derived from human bone marrow, express oct4 and/or telomerase, and have a normal karyotype, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent; and b) determining whether said agent affects said cellular response in said cells of the population. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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Specification