Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof

• US 10,638,734 B2
• Filed: 10/19/2010
• Issued: 05/05/2020
• Est. Priority Date: 01/05/2004
• Status: Expired due to Term

• Current Assignee: ABT Holding Company (Athersys, Inc.)
• Original Assignee: ABT Holding Company (Athersys, Inc.)
• Inventors: Furcht, Leo T., Verfaillie, Catherine M., Reyes, Morayma
• Primary Examiner(s): Berke-Schlessel, David W
• Application Number:
  US12/907,495
• Publication Number:
  US 20110171659A1
• Time in Patent Office: 3,486 Days
• Field of Search: None
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 67/0271 Chimeric vertebrates, e.g. comprising exogenous cells
• CPC Class Codes: C12N 15/873 Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

Abstract

The present invention relates generally to mammalian multipotent adult stem cells (MASC), and more specifically to methods for obtaining, maintaining and differentiating MASC to cells of multiple tissue types. Uses of MASC in the therapeutic treatment of disease are also provided.

Claims

1. A method for identifying an agent that affects a desired cellular response, the method comprising:

a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, express oct4 and/or telomerase and are not tumorigenic, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent;

b) determining whether said agent affects said cellular response in said cells of the population.

2. A method according to claim 1, wherein the cells of the population express telomerase.

3. A method according to claim 1, wherein the cells of the population express oct4.

4. A method according to claim 2, wherein the cells of the population express oct4.

5. A method according to any one of claims 1-3, wherein the cells of the population can differentiate into cell types of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages.

6. A method according to claim 5, wherein the cells of the population can differentiate into at least one cell type of each of the endodermal, ectodermal and mesodermal embryonic lineages.

7. A method according to any one of claims 1-3, wherein the agent is a biological or pharmacological agent.

8. A method according to claim 7, wherein said biological agent is a cytokine, chemokine, or growth factor.

9. A method according to any one of claims 1-3, wherein said cellular response is gene expression.

10. The method of claim 1, wherein the cells that are non-embryonic stem cells, embryonic germ cells, or germ cells have undergone about 30 cell doublings in culture prior to step (a).

11. The method of claim 1, wherein the cells that are not embryonic stem cells, embryonic germ cells, or germ cells, have undergone about 40 cell doublings in culture prior to step (a).

20. The method according to claim 1, where the cells of the population are derived from human bone marrow.

12. A method for identifying an agent that affects a desired cellular response, the method comprising:

a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, are derived from human bone marrow, express oct4 and/or telomerase, and have a normal karyotype, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent; and

b) determining whether said agent affects said cellular response in said cells of the population.

13. A method according to claim 12, wherein the cells of the population express telomerase.

14. A method according to claim 12, wherein the cells of the population express oct4.

15. A method according to claim 13, wherein the cells of the population express oct4.

16. A method according to any one of claims 12-15, wherein the cells of the population can differentiate into cell types of at least two of the endodermal, ectodermal and mesodermal embryonic lineages.

17. A method according to claim 16, wherein the cells of the population can differentiate into at least one cell type of each of the endodermal, ectodermal and mesodermal embryonic lineages.

18. The method of claim 12, wherein the cells that are not non-embryonic stem cells, embryonic germ cells, or germ cells have undergone about 30 cell doublings in culture prior to step (a).

19. The method of claim 12, wherein the cells that are not embryonic stem cells, embryonic germ cells, or germ cells, have undergone about 40 cell doublings in culture prior to step (a).