Methods and compositions involving whey protein isolates
First Claim
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1. A method for fractionating a protein mixture, the method comprising:
- (a) adjusting the pH of the protein mixture based on the isoelectric point of a protein of interest in the protein mixture, thereby rendering a net charge of about zero on the protein of interest;
(b) adjusting conductivity of the protein mixture such that proteins other than the protein of interest are rejected by a charged ultrafiltration membrane; and
(c) contacting said mixture with a charged ultrafiltration membrane to achieve a first permeate and a first retentate, wherein said ultrafiltration membrane has a pore size at least 10×
greater than the molecular mass of at least one of the proteins other than the protein of interest, and has a pore size of between about 86 kDa and about 1,600 KDa, wherein said first permeate comprises an increased ratio of the protein of interest as compared to the protein mixture.
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Abstract
The present invention concerns methods of isolating milk proteins. Methods of the invention include charged ultrafiltration processes that use variations in pH to further separate protein species.
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Citations
17 Claims
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1. A method for fractionating a protein mixture, the method comprising:
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(a) adjusting the pH of the protein mixture based on the isoelectric point of a protein of interest in the protein mixture, thereby rendering a net charge of about zero on the protein of interest; (b) adjusting conductivity of the protein mixture such that proteins other than the protein of interest are rejected by a charged ultrafiltration membrane; and (c) contacting said mixture with a charged ultrafiltration membrane to achieve a first permeate and a first retentate, wherein said ultrafiltration membrane has a pore size at least 10×
greater than the molecular mass of at least one of the proteins other than the protein of interest, and has a pore size of between about 86 kDa and about 1,600 KDa, wherein said first permeate comprises an increased ratio of the protein of interest as compared to the protein mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for separating glycomacropeptide (GMP) from alpha-lactalbumin (ALA), GMP from immunoglobulin G (IgG), GMP from beta-lactoglobulin (BLG), ALA from IgG, or BLG from IgG, the method comprising:
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(a) adjusting pH of a protein mixture comprising two or more of GMP, ALA, GLG, or IgG based on an isoelectric point of one of GMP, ALA, GLG, or IgG present in the protein miture, thereby rendering a net charge of about zero on one of GMP, ALA, GLG, or IgG present in the protein mixture; (b) adjusting conductivity of the protein mixture such that proteins selected from the group consisting of GMP, ALA, BLG, and IgG, other than the protein of step (a) that has a net charge of about zero, are rejected by a charged ultrafiltration membrane; and (c) contacting the protein mixture of step (b) with a charged ultrafiltration membrane tp acjoeve a forst permeate and a first retentate, wherein the ultrafiltration membrane has a pore size at least 10×
greater than the molecular mass of at least one of GMP, ALA, BLG, or IgG, other than the protein of step (a) that has a net charge of about zero, and has a pore size of between about 86 kDa and about 1,600 kDa, wherein the first permeate comprises an increased ratio of the protein of step (a) that has a net charge of about zero as compared to the protein mixture.
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Specification