Mesenchymal stem cell extract and its use

• US 10,639,264 B2
• Filed: 05/15/2018
• Issued: 05/05/2020
• Est. Priority Date: 02/17/2014
• Status: Active Grant

• Current Assignee: Gwo Xi Stem Cell Applied Technology Co., Ltd.
• Original Assignee: Gwo Xi Stem Cell Applied Technology Co., Ltd.
• Inventors: Lin, Shinn-Zong, Harn, Horng-Jyh, Chiou, Tzyy-Wen, Lin, Po-Cheng
• Primary Examiner(s): Nguyen, Nghi V
• Application Number:
  US15/980,184
• Publication Number:
  US 20180250216A1
• Time in Patent Office: 721 Days
• Field of Search: None
• CPC Class Codes: A61F 2007/0003 Face
• CPC Class Codes: A61F 2007/0062 the gas being steam or water vapour
• CPC Class Codes: A61F 2007/0088 Radiating heat
• CPC Class Codes: A61F 7/02 Compresses or poultices for effecting heating or cooling
• CPC Class Codes: A61K 2800/805 Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• CPC Class Codes: A61K 2800/86 Products or compounds obtained by genetic engineering
• CPC Class Codes: A61K 2800/88 Two- or multipart kits
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/981 of mammals or bird
• CPC Class Codes: A61N 5/0625 Warming the body, e.g. hyperthermia treatment
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/14 for baldness or alopecia
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth

Abstract

A mesenchymal stem cell extract and its use are provided, wherein the mesenchymal stem cell extract comprises a trophic factor(s), such as bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF), and/or interleukin-17 (IL-17), and wherein the extract is especially suitable for repairing skin aging.

Claims

1. A mesenchymal stem cell extract, which is a supernatant provided by the following steps:

(1) mixing mesenchymal stem cells separated from an animal fat tissue with a proteolysis buffer containing NP40 lysis buffer, phenylmethanesulfonyl fluoride (PMSF), a protease inhibitor, β

-glycerol phosphate and Na₃VO₄, to lyse the cells and provide a cell lysate; and

(2) centrifugating the cell lysate at a temperature of no more than 4°

C. to collect the supernatant,

wherein the supernatant obtained from step (2) consists of bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF), interleukin-17 (IL-17), other cytoplasmic ingredients and components of the proteolysis buffer.

2. The mesenchymal stem cell extract as claimed in claim 1, which is provided as an emulsion, a cream, a gel, a paste, a spray or a solution.

3. The mesenchymal stem cell extract as claimed in claim 2, wherein the gel is an aquagel.

4. The mesenchymal stem cell extract as claimed in claim 2, wherein the paste is a dispersing paste or an ointment.

5. The mesenchymal stem cell extract as claimed in claim 2, wherein the solution is a washing fluid or a suspension.