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Mesenchymal stem cell extract and its use

  • US 10,639,264 B2
  • Filed: 05/15/2018
  • Issued: 05/05/2020
  • Est. Priority Date: 02/17/2014
  • Status: Active Grant
First Claim
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1. A mesenchymal stem cell extract, which is a supernatant provided by the following steps:

  • (1) mixing mesenchymal stem cells separated from an animal fat tissue with a proteolysis buffer containing NP40 lysis buffer, phenylmethanesulfonyl fluoride (PMSF), a protease inhibitor, β

    -glycerol phosphate and Na3VO4, to lyse the cells and provide a cell lysate; and

    (2) centrifugating the cell lysate at a temperature of no more than 4°

    C. to collect the supernatant,wherein the supernatant obtained from step (2) consists of bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF), interleukin-17 (IL-17), other cytoplasmic ingredients and components of the proteolysis buffer.

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