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Method to treat cancer with engineered T-cells

  • US 10,639,329 B2
  • Filed: 06/12/2016
  • Issued: 05/05/2020
  • Est. Priority Date: 06/12/2015
  • Status: Active Grant
First Claim
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1. A method of treating a human patient having a solid tumor expressing CD19, the method comprising administering to the patient a pharmaceutical composition comprising an anti-tumor effective amount of an autologous T-cell population transduced with one or more lentiviral vectors encoding single or multiple chimeric antigen receptors (CARs), the single or multiple CARs comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NO:

  • 13, SEQ ID NO;

    17, and SEQ ID NO;

    21 to promote in vivo expansion and persistence of the autologous patient-specific anti-tumor T-cells,wherein the T-cells are co-cultured with autologous dendrite cells differentiated from monocytes, the autologous dendrite cells being transduced with one or more lentiviral vectors expressing patient-derived tumor antigens,wherein the T-cell population is additionally transduced with one or more lentiviral vectors encoding tumor-specific T-cell receptors (TCRs) thereby generating an active patient-specific autologous anti-tumor T-cell population capable of recognizing patient-derived tumor antigens which can be infused directly back into the patient to result in tumor stabilization, tumor reduction, elimination of cancer, or remission of cancer in a patient-specific manner.

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