Thermoresponsive bioconjugates and their controlled delivery of cargo
First Claim
1. A method for delivering a thermoresponsive bioconjugate to a target matrix, comprising(a) introducing a thermoresponsive bioconjugate self-assembled into particles to a target matrix, wherein the bioconjugate comprises a first molecule comprising an elastin-like peptide (ELP) having no more than 125 amino acids, a second molecule capable of forming an oligomer, and a linker connecting the first molecule with the second molecule, and wherein the ELP has 3-10 repeats of VPGXG (SEQ ID NO:
- 22), XPGVG (SEQ ID NO;
28) or XPAVG (SEQ ID NO;
27), wherein X is an amino acid, and(b) increasing or decreasing the temperature of the target matrix, whereby the particles are disassembled at the target matrix.
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Abstract
The present invention provides a bioconjugate. The bioconjugate comprises a first molecule comprising an elastin-like peptide (ELP), a second molecule capable of self-associating into an oligomer, and a linker connecting the first molecule with the second molecule. The bioconjugate is self-assembled into particles, which are disassembled when the temperature is increased. Also provided is a method for delivering a bioconjugate to a target matrix, comprising (a) introducing a bioconjugate self-assembled into particles to a target matrix, wherein the bioconjugate comprises a first molecule comprising an elastin-like peptide (ELP), a second molecule capable of self-associating into an oligomer, and a linker connecting the first molecule with the second molecule, and (b) increasing the temperature of the target matrix to disassemble the particles at the target matrix.
6 Citations
20 Claims
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1. A method for delivering a thermoresponsive bioconjugate to a target matrix, comprising
(a) introducing a thermoresponsive bioconjugate self-assembled into particles to a target matrix, wherein the bioconjugate comprises a first molecule comprising an elastin-like peptide (ELP) having no more than 125 amino acids, a second molecule capable of forming an oligomer, and a linker connecting the first molecule with the second molecule, and wherein the ELP has 3-10 repeats of VPGXG (SEQ ID NO: - 22), XPGVG (SEQ ID NO;
28) or XPAVG (SEQ ID NO;
27), wherein X is an amino acid, and(b) increasing or decreasing the temperature of the target matrix, whereby the particles are disassembled at the target matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 19, 20)
- 22), XPGVG (SEQ ID NO;
-
12. A thermoresponsive bioconjugate comprising a first molecule comprising an elastin-like peptide (ELP) having no more than 125 amino acids, a second molecule capable of forming an oligomer, and a linker connecting the first molecule with the second molecule, wherein the ELP has 3-10 repeats of VPGXG (SEQ ID NO:
- 22), XPGVG (SEQ ID NO;
28) or XPAVG (SEQ ID NO;
27), wherein X is an amino acid, wherein the bioconjugate is self-assembled into particles, and wherein the particles are disassembled when the temperature is increased or decreased. - View Dependent Claims (13, 14, 15, 16, 17, 18)
- 22), XPGVG (SEQ ID NO;
Specification