Blood filtration system containing mannose coated substrate

• US 10,639,413 B2
• Filed: 08/02/2018
• Issued: 05/05/2020
• Est. Priority Date: 06/24/2013
• Status: Active Grant

• Current Assignee: ExThera Medical Corporation
• Original Assignee: ExThera Medical Corporation
• Inventors: McCrea, Keith, Ward, Robert, Larm, Olle, Adolfsson, Lars
• Primary Examiner(s): Weiss, Pamela H
• Application Number:
  US16/053,272
• Publication Number:
  US 20190038826A1
• Time in Patent Office: 642 Days
• CPC Class Codes: A61M 1/34 Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
• CPC Class Codes: A61M 1/36 Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
• CPC Class Codes: A61M 1/3673 Anticoagulant coating, e.g. Heparin coating
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 2202/203 Bacteria
• CPC Class Codes: A61M 2205/33 Controlling, regulating or measuring
• CPC Class Codes: A61M 2205/3334 Measuring or controlling the flow rate
• CPC Class Codes: A61M 2205/7518 bacterial
• CPC Class Codes: B01D 39/04 Organic material, e.g. cellulose, cotton
• CPC Class Codes: B01D 39/083 of organic material
• CPC Class Codes: B01D 39/1615 of natural origin
• CPC Class Codes: B01D 63/08 Flat membrane modules
• CPC Class Codes: B01D 63/10 Spiral-wound membrane modules
• CPC Class Codes: B01D 65/08 Prevention of membrane fouling or of concentration polarisation
• CPC Class Codes: B01D 71/08 Polysaccharides
• CPC Class Codes: C07H 1/00 Processes for the preparation of sugar derivatives

Abstract

A blood filtration method, system, device and media for removing gram negative bacteria from the blood wherein the media includes a substrate coated with mannose optionally in constitution with substrate coated with heparin.

Claims

1. A method for attaching a non-reducing mannose to an amine containing substrate, said method comprising:

providing an amine containing substrate;

attaching an intermediate aldehyde to the amine containing substrate;

contacting the amine containing substrate with an aqueous solution containing the non-reducing mannose comprising a reactive amine to form a Schiff base intermediate; and

contacting the Schiff base with a reducing agent to attach said non-reducing mannose.

2. The method of claim 1, wherein said aqueous solution is acidic.

3. The method of claim 1, wherein said amine containing substrate is an aminated bead.

4. The method of claim 1, wherein said reducing agent is a member selected from sodium cyanoborohydride and sodium borohydride.

5. The method of claim 1, wherein said method further comprises reacting heparin having a reactive aldehyde functionality.

6. A bead having attached the non-reducing mannose made according to the method of claim 1.

7. A bead having the non-reducing attached mannose and heparin made according to the method of claim 5.

8. The method of claim 1, wherein said non-reducing mannose is p-aminophenyl-α

-D-mannopyranoside.

9. A method for removing at least one gram negative bacteria from blood comprising:

contacting a sample of blood with the non-reducing mannose substrate according to claim 1.

10. The method of claim 9, wherein the gram negative bacteria is an Enterobacteriaceae.

11. The method of claim 9, wherein the gram negative bacteria is at least one member selected from the group consisting of E. coli, Klebsiella pneumoniae, and P. aeruginosa.

12. A non-reducing mannose attached to an amine containing substrate according to claim 1.