Microfluidic temperature control

• US 10,639,630 B2
• Filed: 01/30/2015
• Issued: 05/05/2020
• Est. Priority Date: 01/30/2015
• Status: Active Grant

• Current Assignee: Hewlett-Packard Development Company, L.P. (HP Inc.)
• Original Assignee: Hewlett-Packard Development Company, L.P. (HP Inc.)
• Inventors: Domingue, Chantelle, Giri, Manish, Bengali, Sadiq
• Primary Examiner(s): Hyun, Paul S
• Application Number:
  US15/545,396
• Publication Number:
  US 20180043356A1
• Time in Patent Office: 1,922 Days
• Field of Search: None
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B81B 7/0087 On-device systems and sensors for controlling, regulating or monitoring

Abstract

A device includes a microfluidic channel structure on a substrate and a first resistive structure on the substrate to control the temperature of at least the substrate. The first resistive structure is separate from, and independent of the, microfluidic channel structure. In some instances, the device includes a second resistive structure.

Claims

1. A biologic test chip comprising:

a substrate;

a microfluidic channel structure on the substrate;

a first resistive structure on the substrate to selectively heat the substrate and to selectively sense a temperature of the substrate to control the temperature of at least the substrate, wherein the first resistive structure is separate from and independent of the microfluidic channel structure;

wherein the first resistive structure comprises a plurality of first resistive structures, and wherein a first one of the first resistive structures has a shape and size generally corresponding to a shape and size of a periphery of the substrate, and

wherein a second one of the first resistive structures has a shape and size generally dissimilar to the shape and size of the periphery of the substrate and is electrically independent from the first one of the first resistive structures.

2. The chip of claim 1, comprising:

a plurality of second resistive structures located in the microfluidic channel structure, at least some of the second resistive structures to selectively pump fluid and to selectively apply heat within the microfluidic channel structure,

wherein the first resistive structure is electrically independent from the second resistive structures.

3. The chip of claim 2, wherein the first resistive structure has a resistivity and a thermal coefficient of resistance that are substantially different than a resistivity and a thermal coefficient of resistance of a second resistive structure of the plurality of second resistive structures.

4. The chip of claim 1, wherein the first resistive structure has lengths and shapes substantially dissimilar from a shape and length of the microfluidic channel structure.

5. The chip of claim 1, wherein the microfluidic channel structure includes a plurality of different microfluidic channel units, wherein a one-to-one correspondence exists between each first resistive structure and a microfluidic channel unit of the plurality of different microfluidic channel units.

6. A biologic microfluidic device comprising:

a substrate;

a microfluidic channel structure on the substrate;

a non-transitory computer-readable medium including instructions operable by a controller;

a plurality of first resistive structures on the substrate separate from and independent of the microfluidic channel structure, wherein the instructions are to operate the first resistive structures to selectively heat the substrate and to selectively sense a temperature of the substrate at a location of each first resistive structure;

a plurality of second resistive structures located in the microfluidic channel structure and electrically independent from the first resistive structures, wherein the instructions are to operate at least some of the second resistive structures to selectively pump fluid and apply heat to the fluid within the microfluidic channel structure without pumping the fluid,

wherein the temperatures sensed via at least some of the first resistive structures provide a feedback loop to at least partially determine whether heat is to be selectively applied to the substrate via at least one of the first resistive structures and at least some of the second resistive structures.

7. The biologic microfluidic device of claim 6, further comprising:

an input/output module in communication with the second resistive structures to enable the controller to make a determination regarding which second resistive structures will apply heat, the determination being made according to locations of the second resistive structures relative to the sensed temperature at the corresponding location of a first resistive structure of the first resistive structures.

8. The biologic microfluidic device of claim 6, comprising:

an input/output module in communication with at least the first resistive structures and the second resistive structures to receive feedback loop information regarding the sensed temperature to enable the controller to initiate a command signal to cause the selective application of heat.

9. The biologic microfluidic device of claim 6, wherein the microfluidic channel structure comprises an array of independent microfluidic channel units, with each independent microfluidic channel unit defining a reaction process independent from reaction processes in other independent microfluidic channel units, wherein a first resistive structure of the first resistive structures corresponds with an independent microfluidic channel unit of the independent microfluidic channel units.

10. A biologic microfluidic system comprising:

a controller;

a biologic microfluidic device comprising;

a substrate;

a microfluidic channel structure on the substrate;

a first resistive array on the substrate separate from and independent of the microfluidic channel structure, wherein the controller is configured to operate the first resistive array to selectively heat the substrate and to selectively sense localized temperatures of the substrate at spaced apart locations on the substrate;

a second resistive array located within the microfluidic channel structure, wherein the controller is configured to operate the second resistive array to selectively pump fluid and apply heat without pumping fluid, the second resistive array being electrically independent from the first resistive array;

an input/output module to communicate a sensed localized temperature of the localized temperatures to the controller for determination of the selective application of heat via at least one of the first resistive array and the second resistive array to achieve a selectively variable temperature protocol in at least one reaction process within the microfluidic channel structure.

11. The biologic microfluidic system of claim 10, wherein the first resistive array comprises a plurality of first resistive elements spaced apart from each other in a pattern at least partially surrounding the microfluidic channel structure.

12. The biologic microfluidic system of claim 11, wherein the second resistive array comprises a plurality of second resistive elements at spaced apart locations within the microfluidic channel structure, wherein a size and shape of at least some of the second resistive elements are substantially different than a size and shape of the microfluidic channel structure.

13. The biologic microfluidic system of claim 10, wherein the controller is configured to operate the first resistive array via the input/output module in a diagnostic mode, prior to intake of a fluid sample within the microfluidic device, to compare a sensed temperature to a reference temperature to determine whether a deviation of the sensed temperature from the reference temperature indicates correct functioning of the biologic microfluidic device.

14. The biologic microfluidic system of claim 10, wherein the controller is configured to operate the second resistive array at a high intensity and short pulse width to pump fluid and at a low intensity and long pulse width to apply heat to the fluid, wherein the low intensity and long pulse width provides insufficient energy to cause fluid displacement.