Nanobodies suitable for neuron regeneration therapy
First Claim
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1. A polypeptide comprising a variable heavy chain domain (VHH) of a camelid heavy-chain antibody,wherein said VHH comprises:
- i) a CDR1 region with the sequence IDVINNMA (SEQ ID NO;
47),ii) a CDR2 region with the sequence TITSGFSTNY (SEQ ID NO;
48), andiii) a CDR3 region with the sequence KVHLIRLGAARAYDY (SEQ ID NO;
49);
wherein the amino acid sequence of said VHH is at least 90% identical to SEQ ID NO;
3; and
wherein said polypeptide is permeable across the blood-brain barrier and has an isoelectric point of at least 8.5.
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Abstract
The invention is in the domain of delivery of molecules to brain cells across the blood-brain barrier. The invention relates to a novel polypeptide-based carrier that allows the efficient delivery of an effector peptide, to neuron cells across the blood-brain barrier, and to methods for the production and testing of such carrier, including a model for testing the capacity of such molecule to cross the blood-brain barrier and/or the toxicity of molecules on the blood brain barrier and/or the capacity of molecules that have crossed to target human brain cells (e.g. neurons, astrocytes and microglial cells).
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Citations
11 Claims
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1. A polypeptide comprising a variable heavy chain domain (VHH) of a camelid heavy-chain antibody,
wherein said VHH comprises: -
i) a CDR1 region with the sequence IDVINNMA (SEQ ID NO;
47),ii) a CDR2 region with the sequence TITSGFSTNY (SEQ ID NO;
48), andiii) a CDR3 region with the sequence KVHLIRLGAARAYDY (SEQ ID NO;
49);wherein the amino acid sequence of said VHH is at least 90% identical to SEQ ID NO;
3; andwherein said polypeptide is permeable across the blood-brain barrier and has an isoelectric point of at least 8.5. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 10)
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8. A polypeptide comprising the amino acid sequence of SEQ ID NO:
- 11.
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11. A polypeptide comprising the amino acid sequence of SEQ ID NO:
- 45.
Specification