Method and culture device for detecting yeasts and molds
First Claim
1. A culture device, comprising:
- a body comprising a self-supporting substrate having a first major surface and a second major surface;
a first adhesive composition disposed on a portion of the first major surface of the substrate;
a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and
a plurality of indicator agents, the plurality of indicator agents comprising;
5-bromo-4-chloro-3-indolyl acetate, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl phosphate para-toluidine salt; and
a cover sheet attached to the body member, whereinthe cover sheet comprises a first major surface facing the body member, andwherein the culture device also comprises a second adhesive composition disposed on a portion of the first major surface of the cover sheet and a substantially dry, cold-water-soluble second hydrogel-forming composition adhered to the second adhesive composition;
wherein at least three of the 5-bromo-4-chloro-3-indolyl acetate, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl phosphate para-toluidine salt are disposed in the first adhesive composition and/or the second adhesive composition.
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Accused Products
Abstract
A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
28 Citations
11 Claims
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1. A culture device, comprising:
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a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents, the plurality of indicator agents comprising; 5-bromo-4-chloro-3-indolyl acetate, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl phosphate para-toluidine salt; and a cover sheet attached to the body member, wherein the cover sheet comprises a first major surface facing the body member, and wherein the culture device also comprises a second adhesive composition disposed on a portion of the first major surface of the cover sheet and a substantially dry, cold-water-soluble second hydrogel-forming composition adhered to the second adhesive composition; wherein at least three of the 5-bromo-4-chloro-3-indolyl acetate, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl phosphate para-toluidine salt are disposed in the first adhesive composition and/or the second adhesive composition. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 11)
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4. The culture device of embodiment 21, further comprising a predefined volume of aqueous liquid disposed between the body member and the coversheet, wherein the aqueous liquid and gelling agent form a hydrogel.
Specification