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Electromechanical lysis of bacterial pathogens using ion concentration polarization

  • US 10,640,744 B2
  • Filed: 10/10/2017
  • Issued: 05/05/2020
  • Est. Priority Date: 06/22/2017
  • Status: Active Grant
First Claim
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1. A method of lysing a cell membrane comprising the steps:

  • a. directing a fluid stream containing cells in a channel comprising an inlet and an outlet and defined, at least in part, by at least a first ion exchange membrane and at least a second ion exchange membrane, wherein the ion exchange membranes are juxtaposed and characterized by the same charge;

    b. applying an electric field across the channel at a voltage and duration sufficient to cause helical electroconvective vortex formation across the channel, thereby lysing the cell membranes of the cells;

    c. collecting an output fluid stream comprising lysate from the outlet; and

    d. isolating the lysate from the output fluid stream.

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