Oligodendroglial cell culturing methods and in methods for treating neurodegenerative disorders by using thyroid hormones or analogues
First Claim
1. A method of differentiating a mammalian oligodendrocyte precursor cell into a mammalian oligodendrocyte, the method comprising culturing the mammalian oligodendrocyte precursor cell in a culture medium comprising 3,5-diiodothyropropionic acid (DITPA) at a concentration which is effective to cause the mammalian oligodendrocyte precursor cell to differentiate into a mammalian oligodendrocyte, wherein the mammalian oligodendrocyte precursor cell was obtained through differentiation from a mammalian embryonic stem cell.
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Abstract
The present invention relates to methods of treating or ameliorating certain neurodegenerative disorders (namely, dysmyelinating and demyelinating disorders) in patients in need of such treatment or amelioration. The invention provides methods of treating or ameliorating a patient in need of such treatment and includes the administration to the patient of: (a) thyroid hormones or thyroid hormone analogues; (b) cell replacement therapies involving the use of homogenous Oligodendrocyte Precursor Cells derived from embryonic stem cells that have been treated with thyroid hormones or thyroid hormone analogues; (c) gene therapy to correct mutated genes in vivo; or (d) a combination of two or more of (a), (b) and (c). The invention also provides compositions and formulations of thyroid hormones and thyroid hormone analogues for use in treating or ameliorating such disorders.
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Citations
10 Claims
- 1. A method of differentiating a mammalian oligodendrocyte precursor cell into a mammalian oligodendrocyte, the method comprising culturing the mammalian oligodendrocyte precursor cell in a culture medium comprising 3,5-diiodothyropropionic acid (DITPA) at a concentration which is effective to cause the mammalian oligodendrocyte precursor cell to differentiate into a mammalian oligodendrocyte, wherein the mammalian oligodendrocyte precursor cell was obtained through differentiation from a mammalian embryonic stem cell.
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9. A human oligodendrocyte precursor cell in a culture medium comprising DITPA at a concentration which is effective to cause the human oligodendrocyte precursor cell to differentiate into a human oligodendrocyte, wherein the human oligodendrocyte precursor cell was obtained through differentiation of a human embryonic stem cell and the human embryonic stem cell is a human embryonic stem cell comprising a transgene encoding GFP operably linked to a Nkx2.1 promoter or a HES3 reporter cell comprising a transgene encoding a GFP.
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10. A cell line comprising a human oligodendrocyte precursor cell in a culture medium comprising DITPA at a concentration which is effective to cause the human oligodendrocyte precursor cell to differentiate into a human oligodendrocyte, wherein the human oligodendrocyte precursor cell was obtained through differentiation of a human embryonic stem cell and the human embryonic stem cell is a human embryonic stem cell comprising a transgene encoding GFP operably linked to a Nkx2.1 promoter or a HES3 reporter cell comprising a transgene encoding a GFP.
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