Ultrasound technology to control the spatial organization of cells and proteins in engineered tissues
First Claim
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1. An in vitro vascularized engineered tissue construct, wherein said construct has a thickness in three-dimensions of at least 2 mm, said construct comprising:
- a polymerized biological support material comprising one or more recombinant fibronectin peptide mimetics;
endothelial cells organized into two or more three-dimensional sheets or columns within different planes of said polymerized biological support material; and
lumen containing neovessels defined by said endothelial cells, said lumen containing neovessels extending within and/or between said three-dimensional sheets or columns of endothelial cells.
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Abstract
The present invention is directed to methods of inducing spatial organization of cells an in vitro culture system using ultrasound technology. The invention is further directed to methods of inducing extracellular matrix remodeling and neovessel formation in an in vitro culture system and generating vascularized engineered tissue constructs using ultrasound technology.
7 Citations
15 Claims
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1. An in vitro vascularized engineered tissue construct, wherein said construct has a thickness in three-dimensions of at least 2 mm, said construct comprising:
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a polymerized biological support material comprising one or more recombinant fibronectin peptide mimetics; endothelial cells organized into two or more three-dimensional sheets or columns within different planes of said polymerized biological support material; and lumen containing neovessels defined by said endothelial cells, said lumen containing neovessels extending within and/or between said three-dimensional sheets or columns of endothelial cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification