Method of producing monomeric card in bacteria
First Claim
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1. A method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria, the method comprising:
- introducing a vector comprising a polynucleotide encoding a caspase (Casp) protein into bacteria to prepare transformed bacteria;
culturing the transformed bacteria to induce expression of caspase;
lysing the cultured bacteria to obtain a lysate; and
obtaining monomeric CARDs from the lysate.
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Abstract
Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc.
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Citations
16 Claims
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1. A method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria, the method comprising:
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introducing a vector comprising a polynucleotide encoding a caspase (Casp) protein into bacteria to prepare transformed bacteria; culturing the transformed bacteria to induce expression of caspase; lysing the cultured bacteria to obtain a lysate; and obtaining monomeric CARDs from the lysate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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