Method of treating pain with an antibody against netrin-4, UNC5B or neogenin

• US 10,640,768 B2
• Filed: 04/10/2018
• Issued: 05/05/2020
• Est. Priority Date: 08/19/2013
• Status: Active Grant

• Current Assignee: Osaka University
• Original Assignee: Osaka University
• Inventors: Yamashita, Toshihide, Hayano, Yasufumi
• Primary Examiner(s): Chong, Kimberly
• Application Number:
  US15/949,330
• Publication Number:
  US 20180230469A1
• Time in Patent Office: 756 Days
• Field of Search: None
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 25/04 Centrally acting analgesics, e.g. opioids
• CPC Class Codes: A61P 29/02 without antiinflammatory effect
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12N 2320/30 Special therapeutic applications
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2500/02 Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
• CPC Class Codes: G01N 2500/10 involving cells
• CPC Class Codes: G01N 2800/2842 Pain, e.g. neuropathic pain, psychogenic pain
• CPC Class Codes: G01N 33/15 Medicinal preparations ; Physical properties thereof, e.g. dissolubility
• CPC Class Codes: G01N 33/6872 Intracellular protein regulatory factors and their receptors, e.g. including ion channels

Abstract

The present invention provides a screening method for pain suppressors, which method is characterized by using netrin-4 and/or a netrin-4 receptor to select a substance capable of inhibiting downstream signaling from netrin-4. According to the screening method of the present invention, pain suppressors useful as a preventive or therapeutic medicine for pain can be identified. The present invention also provides a pharmaceutical composition for prevention or treatment of pain, which composition comprises, as an active ingredient, a substance capable of inhibiting downstream signaling from netrin-4.

Claims

1. A method for treatment of pain, comprising the step of administering, to a mammal, an effective amount of a nucleic acid capable of inhibiting the expression of netrin 4, wherein expression of netrin-4 is inhibited.

2. The method according to claim 1, wherein the nucleic acid is a siRNA composed of nucleotide sequences of SEQ ID NOS:

1 and 2, or nucleotide sequences of SEQ ID NOS;

3 and 4, as sense and antisense strands.

3. A method for treatment of pain, comprising the step of administering, to a mammal, an effective amount of a nucleic acid capable of inhibiting the expression of Unc5B, wherein expression of Unc5B is inhibited.

4. A method for treatment of pain, comprising the step of administering, to a mammal, an effective amount of a nucleic acid capable of inhibiting the expression of neogenin, wherein expression of neogenin is inhibited.

5. The method according to claim 4, wherein the nucleic acid is a siRNA composed of nucleotide sequences of SEQ ID NOS:

5 and 6 as sense and antisense strands.