Method of modulating cell resistance

• US 10,640,778 B2
• Filed: 08/04/2016
• Issued: 05/05/2020
• Est. Priority Date: 08/26/2005
• Status: Active Grant

• Current Assignee: DuPont Nutrition Biosciences ApS (International Flavors & Fragrances Incorporated)
• Original Assignee: DuPont Nutrition Biosciences ApS (International Flavors & Fragrances Incorporated)
• Inventors: Barrangou, Rodolphe, Boyaval, Patrick, Fremaux, Christophe, Horvath, Philippe, Romero, Dennis
• Primary Examiner(s): Marvich, Maria
• Application Number:
  US15/229,103
• Publication Number:
  US 20170037416A1
• Time in Patent Office: 1,370 Days
• Field of Search: None
• CPC Class Codes: A23K 20/10 Organic substances
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: C12N 15/74 Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• CPC Class Codes: C12N 15/746 for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 1/70 involving virus or bacteriophage immunoassay for viruses G01N33/56983
• CPC Class Codes: C12Q 2525/151 repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof.

Claims

1. A DNA construct comprising a cas gene and a heterologous regulatory sequence, which is heterologous to the source of the cas gene, operably linked to at least one nucleotide sequence component of the construct, wherein the cas gene comprises one of the nucleotide sequences of the SEQ ID NO:

s selected from the group consisting of SEQ ID NO;

s 505-508, 510-516, and 518-521 or a cas gene encoding a polypeptide having at least 100% identity to a polypeptide encoded by one of the nucleotide sequences of the SEQ ID NO;

s selected from the group consisting of SEQ ID NO;

s 505-508, 510-516 and 518-521.

2. The construct according to claim 1, comprising one or more CRISPR repeat(s) which are from the same CRISPR locus as the cas gene and originate from a genome or a plasmid.

3. A vector comprising the construct of claim 1.

4. A host cell comprising the construct of claim 1.

5. A cell culture comprising the host cell of claim 4.

6. A cell culture according to claim 5, wherein said culture is a starter culture or a probiotic culture.

7. A food or feed produced from the host cell of claim 4.

8. A construct according to claim 1 wherein said cas gene encodes a Cas protein which keeps the ability to form a functional combination with a CRISPR repeat as set forth in SEQ ID NO:

s 18-19.

9. A DNA construct comprising a cas gene having one of the nucleotide sequences of the SEQ ID NO:

s selected from the group consisting of SEQ ID NO;

s 505-508, 510-516, and 518-521 wherein said cas gene is operably linked to a heterologous regulatory sequence which is heterologous to the source of the cas gene.