Single cycle replicating adenovirus vectors
First Claim
1. A method for inducing an immune response against an antigen in a mammal, wherein said method comprises administering a single cycle replicating adenovirus to said mammal under conditions wherein said adenovirus infects a cell of said mammal and replicates within said cell, wherein said cell does not produce new infectious virions following infection with said adenovirus, wherein said adenovirus comprises an adenovirus polypeptide and lacks at least a portion of a nucleic acid sequence that encodes said adenovirus polypeptide, wherein when said adenovirus lacks at least a portion of a nucleic acid sequence that encodes a fiber protein, said adenovirus comprises a fiber protein having wild-type specificity, wherein said adenovirus comprises a nucleic acid sequence encoding said antigen, and wherein expression of said antigen in said cell leads to induction of said immune response.
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Abstract
This document relates to adenovirus vectors and methods and materials related to using adenovirus vectors. For example, viruses, nucleic acid molecules encoding viruses, cell lines containing viral vectors, and methods for using viruses to deliver nucleic acid to cells in vitro or in vivo are provided. Methods and materials for using adenovirus vectors to induce immune responses and to treat cancer also are provided.
5 Citations
11 Claims
- 1. A method for inducing an immune response against an antigen in a mammal, wherein said method comprises administering a single cycle replicating adenovirus to said mammal under conditions wherein said adenovirus infects a cell of said mammal and replicates within said cell, wherein said cell does not produce new infectious virions following infection with said adenovirus, wherein said adenovirus comprises an adenovirus polypeptide and lacks at least a portion of a nucleic acid sequence that encodes said adenovirus polypeptide, wherein when said adenovirus lacks at least a portion of a nucleic acid sequence that encodes a fiber protein, said adenovirus comprises a fiber protein having wild-type specificity, wherein said adenovirus comprises a nucleic acid sequence encoding said antigen, and wherein expression of said antigen in said cell leads to induction of said immune response.
- 7. A method for delivering a therapeutic polypeptide to a mammal, wherein said method comprises administering a single cycle replicating adenovirus to said mammal under conditions wherein said adenovirus infects a cell of said mammal and replicates within said cell, wherein said cell does not produce new infectious virions following infection with said adenovirus, wherein said adenovirus comprises an adenovirus polypeptide and lacks at least a portion of a nucleic acid sequence that encodes said adenovirus polypeptide, wherein when said adenovirus lacks at least a portion of a nucleic acid sequence that encodes a fiber protein, said adenovirus comprises a fiber protein having wild-type specificity, wherein said adenovirus comprises a nucleic acid sequence encoding said therapeutic polypeptide.
Specification