Devices and methods using modified paper electrodes for the detection of hemoglobin A1C and glucose

• US 10,640,801 B2
• Filed: 09/26/2016
• Issued: 05/05/2020
• Est. Priority Date: 09/25/2015
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Li, XiuJun, Henao-Pabon, Gilberto
• Primary Examiner(s): Underdahl, Thane
• Application Number:
  US15/276,408
• Publication Number:
  US 20170088875A1
• Time in Patent Office: 1,317 Days
• Field of Search: None
• CPC Class Codes: C07F 5/025 Boronic and borinic acid compounds
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 2021/7769 Measurement method of reaction-produced change in sensor
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin

Abstract

Certain embodiments are directed to sensors that enable the use of optimized biocompatible materials such as pre-anodized paper printed electrode transducer to detect binding of a target agent, wherein the surface is modified or functionalized through zero length cross-linker so that it interacts with or specifically binds a target such as sugars (glucose) or glycated proteins (HgbA1c).

Claims

1. A glycated hemoglobin sensor comprising a conductive carbon ink printed electrode on a paper support having a functionalized surface coupled to a sugar reactive moiety, wherein the sugar reactive moiety binds glycated hemoglobin and is 3-AminoPhenyl-Boronic Acid (APBA) and wherein the conductive carbon contains surface carbonyl functionalities on edge plane sites.

2. A diagnostic device comprising the sensor of claim 1.

3. A method of detecting glycated hemoglobin comprising (i) contacting a sensor of claim 1 with a sample to form an exposed sensor, and (ii) contacting the exposed sensor with a glycated hemoglobin detection reagent that produces a detectable signal in the presence of hemoglobin.

4. The method of claim 3, wherein the detection reagent is H₂O₂.

5. The method of claim 3, wherein the detectable signal is an electric signal.

6. The method of claim 5, wherein the signal is generated by direct electron transfer.

7. The method of claim 3, wherein the sample is blood or a blood fraction.