Method and kit for detecting target nucleic acid
First Claim
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1. A method for detecting a target nucleic acid, comprising the steps of:
- (a) amplifying the target nucleic acid by reacting a sample comprising the target nucleic acid with a labeled primer comprising (i) a binding region complementary to the target nucleic acid and (ii) a nucleic acid oligomer comprising nucleotides non-complementary to the target nucleic acid, and (iii) a label;
(b) reacting a product amplified in step (a) with a membrane having immobilized thereon a probe that binds complementarily to the nucleic acid oligomer; and
(c) detecting whether the label has been reacted in step (b), wherein the reacted label is indicative of amplification of the target nucleic acid, andwherein the nucleic acid oligomer comprises one or more nucleotide sequences selected from the group consisting of SEQ ID NOS;
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Abstract
The present disclosure relates to a method and a kit for analyzing a nucleic acid amplification product, and more particularly to a method and kit of analyzing a nucleic acid amplification product using a labeled primer comprising (i) a binding region complementary to the target nucleic acid, (ii) a nucleic acid oligomer containing nucleotides non-complementary to the target nucleic acid, and (iii) a label, and using a membrane having immobilized thereon a probe that binds complementarily to the nucleic acid oligomer.
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9 Claims
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1. A method for detecting a target nucleic acid, comprising the steps of:
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(a) amplifying the target nucleic acid by reacting a sample comprising the target nucleic acid with a labeled primer comprising (i) a binding region complementary to the target nucleic acid and (ii) a nucleic acid oligomer comprising nucleotides non-complementary to the target nucleic acid, and (iii) a label; (b) reacting a product amplified in step (a) with a membrane having immobilized thereon a probe that binds complementarily to the nucleic acid oligomer; and (c) detecting whether the label has been reacted in step (b), wherein the reacted label is indicative of amplification of the target nucleic acid, and wherein the nucleic acid oligomer comprises one or more nucleotide sequences selected from the group consisting of SEQ ID NOS;
1-3. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification