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Methods of identifying nucleic acids from a single cell using an electrical cell-trapping array

  • US 10,640,824 B1
  • Filed: 05/30/2017
  • Issued: 05/05/2020
  • Est. Priority Date: 06/03/2016
  • Status: Active Grant
First Claim
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1. A method of identifying nucleic acids of interest within single cells, comprising:

  • contacting a solid surface with a plurality of cells wherein the solid surface comprises an array of identical features, wherein each identical feature comprises a library of distinct oligonucleotides, wherein each oligonucleotide comprises at least one unique molecular identifier sequence and a sequence complimentary to one of a plurality of nucleic acids of interest, wherein the surface further comprises a planar electrode of negative charge and wherein the planar electrode is positioned over the solid surface with space between the solid surface and planar electrode of negative charge;

    immobilizing at least one of the cells at one of the identical features on the array on the solid surface by application of an electric field;

    releasing the nucleic acids of interest from the cells;

    hybridizing the oligonucleotides to the nucleic acids of interest;

    releasing the hybridized nucleic acids of interest and oligonucleotides from the solid surface;

    sequencing the released hybridized nucleic acids of interest and oligonucleotides to generate sequence data; and

    analyzing the sequence data to identify nucleic acids of interest within the single cells.

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