Low sequence bias single-stranded DNA ligation
First Claim
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1. A composition comprising a donor nucleic acid molecule, wherein the molecule comprises a stem-loop structure and a 3′
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure, wherein the donor molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO;
5 through SEQ ID NO;
22, SEQ ID NO;
28 and SEQ ID NO;
29.
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Abstract
The invention provides compositions and methods for ligating single stranded nucleic acids wherein the ligation is based on fast, efficient, and low-sequence bias hybridization of an acceptor molecule with a donor molecule. In one embodiment, the structure of the donor molecule comprises a stem-loop intramolecular nucleotide base pairing (i.e., hairpin) and a 3′-overhang region such that the overhang is able to hybridize to nucleotides present in the 3′ end of the acceptor molecule.
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Citations
9 Claims
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1. A composition comprising a donor nucleic acid molecule, wherein the molecule comprises a stem-loop structure and a 3′
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure, wherein the donor molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO;
5 through SEQ ID NO;
22, SEQ ID NO;
28 and SEQ ID NO;
29. - View Dependent Claims (2, 3, 4, 5, 6)
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure, wherein the donor molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO;
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7. A kit comprising a donor nucleic acid molecule, wherein the molecule comprises a stem-loop structure and a 3′
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure, wherein the donor molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO;
5 through SEQ ID NO;
22, SEQ ID NO;
28 and SEQ ID NO;
29. - View Dependent Claims (8, 9)
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure, wherein the donor molecule comprises a nucleotide sequence selected from the group consisting of SEQ ID NO;
Specification