Rapid detection of infectious agents
First Claim
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1. A method for detecting a target nucleic acid that may be present in a sample, said method comprising the steps of:
- a) contacting nucleic acids from said sample with amplification reagents comprising a DNA polymerase, nucleotide monomers, and two or more different primers specifically hybridizing to said target nucleic acid for generating an amplicon, wherein each of said primers comprise at least 10 contiguous nucleotides selected from SEQ ID NOs;
2-4, 9-10, 12, 14-23, 25-26, and 28-32 and performing an amplification reaction by incubating said nucleic acids with said amplification reagents for a period of time under conditions sufficient to amplify said target nucleic acid; and
b) detecting the target nucleic acid by detecting an amplicon from said amplification reaction;
wherein the presence of said amplicon indicates the presence of said target nucleic acid in said sample.
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Abstract
The invention generally relates to a method for detecting a target nucleic acid in a sample. This invention is useful for detecting bacterial or viral agents in a sample, and is able to detect nucleic acids from a broad variety of, e.g., bacteria, rather than only one or a few different bacteria at a time.
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Citations
16 Claims
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1. A method for detecting a target nucleic acid that may be present in a sample, said method comprising the steps of:
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a) contacting nucleic acids from said sample with amplification reagents comprising a DNA polymerase, nucleotide monomers, and two or more different primers specifically hybridizing to said target nucleic acid for generating an amplicon, wherein each of said primers comprise at least 10 contiguous nucleotides selected from SEQ ID NOs;
2-4, 9-10, 12, 14-23, 25-26, and 28-32 and performing an amplification reaction by incubating said nucleic acids with said amplification reagents for a period of time under conditions sufficient to amplify said target nucleic acid; andb) detecting the target nucleic acid by detecting an amplicon from said amplification reaction;
wherein the presence of said amplicon indicates the presence of said target nucleic acid in said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification