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Method for detecting mycoplasma

  • US 10,640,834 B2
  • Filed: 09/07/2015
  • Issued: 05/05/2020
  • Est. Priority Date: 09/10/2014
  • Status: Active Grant
First Claim
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1. A kit for detecting Mycoplasma in a test sample by a multiplex real time quantitative PCR, whereinthe kit comprises one or more forward primers, two or more reverse primers, one or more probes and a solid support;

  • the probe(s) is immobilized onto the solid support;

    the probe is a probe for specifically detecting products amplified by use of the forward primer and at least any one of the reverse primers;

    the forward primer(s) is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence of SEQ ID No;

    1, and which contains a nucleotide sequence (caaggtatccs) at nucleotide positions 14 to 24 in SEQ ID No;

    1;

    the reverse primers each are an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in one or more nucleotide sequences of SEQ ID Nos;

    14 and 17 to 20; and

    the probe(s) is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence of SEQ ID No;

    33, or which consists of a complementary nucleotide sequence thereto.

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