Separation of human papillomavirus nucleic acids from biological samples
First Claim
Patent Images
1. A mixture of at least two oligomers in which individual oligomer sequences are selected from the group consisting of SEQ ID NOs:
- 1 to 10, which includes the complementary oligomer sequences or RNA equivalents of the specified sequences, wherein the mixture includes;
at least two oligomers selected from SEQ ID NOs;
2, 4, 6, 8 and 10 with a ligand moiety joined to each oligomer, wherein at least one of the at least two oligomers is SEQ ID NO;
4 or 10 joined to the ligand moiety;
oligomers of SEQ ID NOs;
2, 4, 6, 8 and 10 with a ligand moiety joined to each oligomer;
at least two oligomers selected from SEQ ID Nos. 1, 3, 5, 7 and 9, wherein at least one of the at least two oligomers is SEQ ID NO;
3 or 9;
oroligomers of SEQ ID NOs;
1, 3, 5, 7 and 9.
2 Assignments
0 Petitions
Accused Products
Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
87 Citations
13 Claims
-
1. A mixture of at least two oligomers in which individual oligomer sequences are selected from the group consisting of SEQ ID NOs:
- 1 to 10, which includes the complementary oligomer sequences or RNA equivalents of the specified sequences, wherein the mixture includes;
at least two oligomers selected from SEQ ID NOs;
2, 4, 6, 8 and 10 with a ligand moiety joined to each oligomer, wherein at least one of the at least two oligomers is SEQ ID NO;
4 or 10 joined to the ligand moiety;oligomers of SEQ ID NOs;
2, 4, 6, 8 and 10 with a ligand moiety joined to each oligomer;at least two oligomers selected from SEQ ID Nos. 1, 3, 5, 7 and 9, wherein at least one of the at least two oligomers is SEQ ID NO;
3 or 9;
oroligomers of SEQ ID NOs;
1, 3, 5, 7 and 9. - View Dependent Claims (2, 3, 6, 7, 8, 9, 10)
- 1 to 10, which includes the complementary oligomer sequences or RNA equivalents of the specified sequences, wherein the mixture includes;
-
4. A method of separating a human papillomavirus (HPV) nucleic acid from other components present within a biological sample, comprising the steps of:
contacting nucleic acid in a biological sample containing RNA of at least one of HPV types 33, 51, 52, 56, 58, and 66 with a capture oligomer comprising an oligomer sequence selected from the group consisting of SEQ ID NOs;
3, 4, 9, and 10, which includes the complementary oligomer sequences or RNA equivalents of the specified sequences.- View Dependent Claims (5, 11, 12, 13)
Specification