Holographic detection and characterization of large impurity particles in precision slurries

• US 10,641,696 B2
• Filed: 09/15/2016
• Issued: 05/05/2020
• Est. Priority Date: 09/18/2015
• Status: Active Grant

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Ruffner, David B., Grier, David G., Philips, Laura
• Primary Examiner(s): Lee, Hwa Andrew
• Application Number:
  US15/759,502
• Publication Number:
  US 20180252628A1
• Time in Patent Office: 1,328 Days
• CPC Class Codes: G01N 15/0227 using imaging; using holography
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0053 in liquids, e.g. trouble
• CPC Class Codes: G01N 2015/0233 using holography
• CPC Class Codes: G01N 2015/1454 using phase shift or interference, e.g. for improving contrast
• CPC Class Codes: G01N 2015/1493 Particle size
• CPC Class Codes: G01N 21/453 Holographic interferometry for dimensional measurements G01B9/021 - G01B9/029
• CPC Class Codes: G03H 1/0005 Adaptation of holography to specific applications holographic optical element G02B5/32; holographic scanner G02B26/106; recognition using holographic mask G06V10/88; holographic memories G11B7/0065, G11C13/042
• CPC Class Codes: G03H 1/0443 Digital holography, i.e. recording holograms with digital recording means holobject computation G03H1/0866
• CPC Class Codes: G03H 2001/0033 in hologrammetry for measuring or analysing
• CPC Class Codes: G03H 2001/005 in microscopy, e.g. digital holographic microscope [DHM]
• CPC Class Codes: G03H 2001/0447 In-line recording arrangement

Abstract

Impurities within a sample are detected by use of holographic video microscopy. The sample flows through the microscope and holographic images are generated. The holographic image is analyzed to identify regions associated with large impurities in the sample. The contribution of the particles of the sample to the holographic images is determined and the impurities are characterized.

Claims

1. A method of characterizing impurities in a sample of a slurry of particles a fluid, comprising:

flowing the slurry through an observation volume of a holographic microscope;

generating a first holographic image, by a holographic video microscopy system having a laser, of the sample within the observation volume at a first time;

analyzing the first holographic image for one or more regions of interest corresponding to a particle of interest;

normalizing the region of interest for a contribution of a diffuse wave created by interaction of the laser with the slurry;

fitting the normalized region of interest to a light scattering theory; and

characterizing, based upon the fitting of the normalized region of interest, one or more properties of particle of interest;

wherein a depth of the observation volume is less than the attenuation depth of the laser in the slurry.

2. The method of claim 1, wherein the slurry comprises particles of 200 nm or less size.

3. The method of claim 2, wherein the particle of interest has a size of about 200 nanometers to about 20 micrometers.

4. The method of claim 1, wherein the peak flow is about 100 micrometers per second.

5. The method of claim 1, further comprising selecting a sample cell of depth D.

6. The method of claim 1, wherein characterizing the one or more particles of interest comprises determining radius and refractive index.

7. A method of characterizing particles of interest in a slurry, comprising:

flowing the slurry through an observation volume of a holographic microscope, the slurry comprising slurry particles in a fluid and particles of interest;

interacting a laser beam with a sample of the slurry and generating a first holographic image based upon holographic video microscopy of the sample within the observation volume at a first time, the first holographic image recording a light field from a scattered wave, generated by interaction of the laser beam with a particle of the particles of interest, and a diffuse wave, generated by interaction of the laser beam and the scattered wave with the slurry particles;

quantifying a contribution of the diffuse wave to the first holographic image;

applying Lorenz Mie analysis to the first holographic image; and

characterizing, based upon the Lorenz Mie analysis, the particle of the particles of interest.

8. The method of claim 7, wherein the slurry comprises particles of 200 nm or less size.

9. The method of claim 8, wherein the particle of interest has a size of about 200 nanometers to about 20 micrometers.

10. The method of claim 7, wherein the peak flow is about 100 micrometers per second.

11. The method of claim 7, further comprising selecting a sample cell of depth D.

12. The method of claim 7, wherein characterizing the particle of interest comprises determining radius and refractive index.

13. The method of claim 7, further comprising, after quantifying the contribution, normalizing the region of interest for the contribution of the diffuse wave.